茉莉花全基因组SSR标记开发及其亲缘关系鉴定  

SSR Molecular Markers Development and Parentage Relationship Identification Based on Whole Genomic Sequences of Jasminum sambac

作　　者：李春牛[1] 苏群 李先民[1] 黄展文 孙明艳 卢家仕[1] 王虹妍 卜朝阳[1] LI Chunniu;SU Qun;LI Xianmin;HUANG Zhanwen;SUN Mingyan;LU Jiashi;WANG Hongyan;and BU Zhaoyang(Flower Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China)

机构地区：[1]广西农业科学院花卉研究所,南宁530007

出　　处：《园艺学报》2024年第10期2343-2357,共15页Acta Horticulturae Sinica

基　　金：广西自然科学基金项目(2020GXNSFAA297190);广西农业科学院科技发展基金项目(桂农科2023JZ12);广西重点研发计划项目(桂科AB18221064);广西科技基地和人才专项(桂科AD17195065)。

摘　　要：根据茉莉花(Jasminum sambac)全基因组序列进行SSR分子标记开发,分析其SSR位点的组成及特征,并开展40份素馨属(Jasminum)种质的遗传多样性分析及41份茉莉花开放授粉系的亲缘关系鉴定。利用MISA软件对单、双瓣茉莉花全基因组进行SSR位点检索,共检测到140803个,重复基元中共有5种重复类型,以二核苷酸和三核苷酸的重复次数最多,分别为87785次和46735次,占SSR总位点数的62.35%和33.19%。挑选单、双瓣茉莉花共有且存在变异的SSR位点,根据单瓣茉莉花的参考基因组,采用Primer v3.0软件成功设计出1847对SSR引物,随机挑选出均匀分布的240对引物,以8个遗传背景差异较大的茉莉花种质混合DNA为模板对其进行扩增,最终确定了14对多态性SSR标记。利用开发的14对SSR标记对40份茉莉花种质进行检测,共发现169个等位基因,平均等位基因数(Na)、有效等位基因(Ne)、Shannon’s信息指数(I)、观察杂合度(Ho)和期望杂合度(He)分别为12.1、4.067、1.772、0.505和0.725,多态性信息含量(PIC)介于0.378~0.817之间,均值为0.702。通过UPGMA聚类,在Nei’s遗传距离0.82处将40份茉莉花种质划分为3大类群;在遗传距离0.66处,茉莉花和毛茉莉(J.multiflorum)聚为一类。群体结构分析表明40份茉莉花种质可分为3个类群,群体间存在部分种质混杂。利用开发的SSR标记对41份开放授粉系及14份候选父本进行检测,共发现103个等位基因,平均Na、Ne、I、Ho和He分别为7.4、2.446、1.061、0.366和0.522,PIC介于0.159~0.744之间,均值为0.477,有6对引物为高度多态位点;在Nei’s遗传距离0.82处将55份供试材料划分为3大类群,供试茉莉花母本及其开放授粉系全部聚为一类,在遗传距离0.28处2个茉莉花母本分别与其开放授粉系聚在一起。利用Cervus v3.07软件分析SSR位点在开放授粉系和已知母本及候选父本的遗传多样性参数,位点累积排除概率随位点数增加而增大,对于�This research is the first time developing SSR molecular markers based on whole genomic sequences of Jasminum sambac,analyzing the composition and characteristics of SSR loci in the whole genomic of J.sambac,and conducting genetic diversity analysis of 40 collected Jasminum germplasms and parentage relationship identification of 41 open pollination lines of J.sambac.The research results were as follows:a total of 140803 SSR loci were detected in the whole genome sequences of single and double petal J.sambac using MISA software.There are five types of repeat motifs,the highest number of repeat types are dinucleotides and trinucleotides,87785 and 46735 respectively,accounting for 62.35%and 33.19%of the total SSR loci.The SSR loci that are common and have variations in single and double petal J.sambac are selected based on the reference genome of single petal J.sambac,1847 pairs of primers were successfully designed using Primer v3.0 software,and 240 pairs of evenly distributed SSR primers were randomly selected.The mixed DNA of 8 Jasminum germplasms with significant genetic background differences was used as the template for amplification,and 14 pairs of polymorphic SSR markers were finally developed.Using the developed SSR markers,a total of 169 alleles were detected in 40 Jasminum germplasm materials.The average number of alleles per locus(Na),effective number of alleles(Ne),Shannon’s Information Index(I),observed heterozygosity(Ho)and expected heterozygosity(He)were 12.1,4.067,1.772,0.505 and 0.725,respectively.The polymorphism information content(PIC)ranged from 0.378 to 0.817,with a mean of 0.702.Through UPGMA clustering,40 Jasminum germplasms were divided into three major groups at Nei’s genetic distance of 0.82.J.sambac and J.multiflorum were separately clustered into one group with Nei’s genetic distance of 0.66.Genetic structure analysis shows that 40 Jasminum germplasms can be divided into 3 groups,and there are some genetic exchange between groups.41 open pollination lines and 14 candidate male par

关 键 词：茉莉花 SSR分子标记 亲缘鉴定 遗传多样性 全基因组 

分 类 号：S685.16[农业科学—观赏园艺]