黄芪多糖APS-Ⅱ在M细胞上的转运吸收机制初探  

Preliminary study of the transport and absorption mechanism of Astragalus polysaccharide-Ⅱon M cells

作　　者：吕弯弯 李科[1,2,3,4] 冯仕红 文雨薇 秦雪梅 杜昱光[4] 李震宇 LÜWan-wan;LI Ke;FENG Shi-hong;WEN Yu-wei;QIN Xue-mei;DU Yu-guang;LI Zhen-yu(Modern Research Center for Traditional Chinese Medicine of Shanxi University,Taiyuan 030006,China;Key Laboratory of Chemical Biology and Molecular Engineering,Ministry of Education of Shanxi University,Taiyuan 030006,China;Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province,Taiyuan 030006,China;Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China)

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学生物学与分子工程教育部重点实验室,山西太原030006 [3]地产中药功效物质研究与利用山西省重点实验室,山西太原030006 [4]中国科学院过程工程研究所,北京100190

出　　处：《药学学报》2024年第10期2820-2827,共8页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81872962);国家博士后科学基金资助项目(2019M650851);国家重点研发计划(2019YFC1710800);山西省重点研发计划重点项目(201603D311101);山西省优秀人才科技创新项目(201605D211030,201705D211020);山西省科技创新人才团队专项基金。

摘　　要：通过建立M细胞模型,探究黄芪多糖APS-Ⅱ在体内的吸收机制。首先将黄芪多糖(Astragalus polysaccharides,APS)通过超滤法分为2种不同相对分子质量多糖APS-I(>2000 kDa)和APS-II(10 kDa),并制备出黄芪多糖APS-Ⅱ(10 kDa),然后对其进行荧光标记;同时通过Caco-2细胞和Raji细胞构建M细胞模型,并对其进行模型验证。采用转运抑制剂对M细胞模型进行处理,探究黄芪多糖APS-Ⅱ在M细胞上的转运情况。结果显示,通过结构与活性验证FITC已成功标记到了APS-Ⅱ的末端,同时M细胞模型构建成功,并发现APS-Ⅱ可以被M细胞所转运,通过5-(N-乙基-N-异丙基)阿米洛利(EIPA)、染料木素(genistein)、dynasore和诺考达唑(nocodazole)4种转运抑制剂说明APS-Ⅱ可能通过网格蛋白和小窝蛋白介导的内吞作用进入细胞。To explore the absorption mechanism of APS-Ⅱin vivo by establishing M cell model.First,Astragalus polysaccharides(APS)was divided into two different molecular weight polysaccharides APS-I(>2000 kDa)and APS-II(10 kDa)by ultrafiltration,and APS-II(10 kDa)was prepared and fluorescently labeled.Meanwhile,M cell model was constructed by Caco-2 cells and Raji cells.The M cell model was treated with transport inhibitors to explore the transport of APS-Ⅱon M cells.The results show that FITC has been successfully labeled to the end of APS-Ⅱ,and the M cell model was successfully constructed,which found that APS-Ⅱcould be transported by M cells,and four transport inhibitors of 5-(N-ethyl-N-isopropyl)amiloride(EIPA),genistein,dynasore and nocodazole indicated that APS-II may enter cells through clathrin and caveolin-mediated endocytosis.

关 键 词：黄芪多糖APS-Ⅱ M细胞 转运抑制剂 吸收机制 

分 类 号：R917[医药卫生—药物分析学]