基于转录组测序的蓝光补光调控三叶青叶片酚类成分生物合成途径分析  

Analysis of the biosynthesis pathways of phenols in the leaves of Tetrastigma hemsleyanum regulated by supplemental blue light based on transcriptome sequencing

作　　者：徐惠龙[1] 杨楠 洪玉燕 潘梦婷 郭玉春 范世明[1] 许文[1] XU Hui-long;YANG Nan;HONG Yu-yan;PAN Meng-ting;GUO Yu-chun;FAN Shi-ming;XU Wen(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建农林大学农学院,福建福州350002

出　　处：《药学学报》2024年第10期2864-2870,共7页Acta Pharmaceutica Sinica

基　　金：福建省科技厅项目(2016N0015,2018N0012,2021Y4015,2021S_(2)015,2022N5018);福建中医药大学校管课题(X2021013-重点;X2023031);厦门市科技特派员专项创新服务载体项目(2022);国家中医药管理局2023年中央财政转移支付地方项目《中药资源保障能力提升项目》。

摘　　要：分析三叶青叶片蓝光补光下酚类成分及基因表达谱的变化情况,筛选酚类代谢相关差异表达基因,为提高三叶青叶品质提供依据。以蓝光补光和可见光的三叶青叶片为研究材料,蓝光补光可以显著提高新绿原酸、绿原酸和3-O-香豆酰奎宁酸等酚类成分含量。转录组共获得102949条unigenes,其中有14564条差异表达unigenes。通过GO功能可分为52个亚类,而KEGG富集分析显示差异表达基因显著富集在苯丙烷生物合成、黄酮类生物合成和异黄酮生物合成等酚类代谢途径中。在三叶青酚类成分生物合成相关通路中共鉴别出63条差异表达基因,包含苯丙氨酸氨裂解酶(phenylalanine ammonia-lyase,PAL)、肉桂醇脱氢酶(cinnamyl-alcohol dehydrogenase,CAD)和黄酮醇合成酶(flavonol synthase,FLS)等19种关键酶。本研究丰富了三叶青的遗传数据信息,为解析蓝光促进三叶青酚类成分生物合成机制、开展三叶青分子育种奠定基础。Analyze the changes in phenolic components and gene expression profiles of Tetrastigma hemsleyanum leaves with supplemental blue light,and screen differentially expressed genes related to phenolic metabolism,providing a basis for improving the quality of T.hemsleyanum leaves.Using the leaves of T.hemsleyanum under supplemental blue light and visible light as research materials,the content of phenolic components such as neochlorogenic acid,chlorogenic acid,and 3-O-coumaroyl quinic acid was significantly increased by supplemental blue light.A total of 102949 unigenes were obtained from the transcriptome,including 14564 differentially expressed unigenes.They can be divided into 52 subclasses by gene ontology(GO)function,kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis showed that these differentially expressed unigenes were significantly enriched in phenolic metabolic pathways such as phenylpropane biosynthesis,flavonoid biosynthesis and isoflavone biosynthesis.A total of 63 differentially expressed unigenes were identified in the pathways related to the biosynthesis of phenolic cpmponents,including 19 key enzymes such as phenylalanine ammonia-lyase(PAL),cinnamyl-alcohol dehydrogenase(CAD),flavonol synthase(FLS),and so on.This study greatly enriched the genetic data information of T.hemsleyanum and laid a foundation for analyzing the mechanism of blue light promoting the biosynthesis of phenols and molecular breeding of T.hemsleyanum.

关 键 词：三叶青 转录组 蓝光补光 差异表达 酚类合成 

分 类 号：R931[医药卫生—生药学]