络石内生菌Myrothecium roridum产生的一个新单端孢霉烯大环内酯  

A new trichothecene macrolide from endophyte Myrothecium roridum associated with Trachelospermum jasminoides

作　　者：韩旭 李晓雯[1] 张凤 方蕾[1] 申丽[1,2] HAN Xu;LI Xiao-wen;ZHANG Feng;FANG Lei;SHEN Li(Medical College of Yangzhou University,Yangzhou 225009,China;Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases,Yangzhou 225009,China)

机构地区：[1]扬州大学医学院,江苏扬州225009 [2]江苏省中西医结合老年病防治重点实验室,江苏扬州225009

出　　处：《药学学报》2024年第10期2889-2893,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金项目(21372191);扬州市重点研发项目(社会发展)(YZ2023068)。

摘　　要：采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及高效液相色谱法从络石(Trachelospermum jasminoides)内生菌Myrothecium roridum IFB-E008发酵液的粗浸膏中分离得到3个化合物。经高分辨质谱、一维和二维核磁共振谱及文献比对等方法,鉴定为:3′-iso-isororidin A(1)、verrol(2)和N-乙酰色胺(3),其中,3′-iso-isororidin A(1)是一个未见文献报道的新单端孢霉烯大环内酯。体外细胞毒活性测定表明,化合物2对人胃癌细胞株SGC-7901具有一定的抑制活性,半数抑制浓度(half inhibition concentration,IC_(50))为59.79μg·m L^(-1)(158.1μmol·L^(-1)),阳性对照顺铂的IC_(50)值为6.58μg·m L^(-1)(21.9μmol·L^(-1))。Three compounds were obtained from the crude extract of the fermentation broth of endophyte Myrothecium roridum IFB-E008 in Trachelospermum jasminoides by silica gel column chromatography,Sephadex LH-20 gel column chromatography and high performance liquid chromatography.They were determined as 3′-isoisororidin A(1),verrol(2)and N-acetyltryptamine(3),respectively,through HR-MS,1D and 2D NMR and literature comparison.3′-iso-isororidin A(1)is a new trichothecene macrolide that has never been reported in the literature.The in vitro cytotoxicity assay showed that compound 2 had certain cytotoxicity against the human gastric cell line SGC-7901 with half inhibition concentration(IC_(50))of 59.79μg·mL^(-1)(158.1μmol·L^(-1)),while IC_(50) value of the positive control cisplatin was 6.58μg·mL^(-1)(21.9μmol·L^(-1)).

关 键 词：络石 内生菌 Myrothecium roridum 次生代谢产物 单端孢霉烯 

分 类 号：R931[医药卫生—生药学]