含白藜芦醇培养基培养的人眼结膜和胸部皮肤病理性瘢痕成纤维细胞增殖凋亡侵袭迁移情况观察  

Proliferation,apoptosis,invasion,and migration of fibroblasts in human conjunctiva and thoracic skin pathological scars cultured in resveratrol-containing medium

作　　者：张艺龄 唐志铭 乔磊 宫朝举 李美丽 庞昆[1] 丁继存 ZHANG Yiling;TANG Zhiming;QIAO Lei;GONG Chaoju;LI Meili;PANG Kun;DING Jicun(Xuzhou Central Hospital,Xuzhou 221009,China;不详)

机构地区：[1]徐州市中心医院徐州医学院徐州临床医学院,江苏徐州221009 [2]徐州市中医院皮肤科 [3]徐州医科大学附属徐州市立医院徐州市第一人民医院

出　　处：《山东医药》2024年第27期40-44,共5页Shandong Medical Journal

基　　金：江苏省中医药科技发展计划项目(MS2021051);徐州市科技项目(KC21262、KC22160);徐州医科大学附属医院发展基金(XYFM202317);中国博士后基金面上项目(2022M722674);徐州市医学后备人才计划项目(XWRCHT20220009)。

摘　　要：目的观察含白藜芦醇的培养基培养的人眼结膜翼状胬肉(眼结膜病理性瘢痕)成纤维细胞和人胸部皮肤病理性瘢痕(皮肤病理性瘢痕)成纤维细胞增殖凋亡侵袭迁移情况,以探讨白藜芦醇对人眼结膜和皮肤病理性瘢痕成纤维细胞增殖凋亡侵袭迁移的作用。方法采用组织块贴壁法分离原代人眼结膜和皮肤病理性瘢痕成纤维细胞。用含0(对照)、1、2、4、8、16、32、64、128、256μg/mL白藜芦醇的培养基培养48 h时,采用CCK-8法观察人眼结膜和皮肤病理性瘢痕成纤维细胞的细胞活力(OD值)。用含0、75μg/mL白藜芦醇的培养基培养人眼结膜和皮肤病理性瘢痕成纤维细胞8 h,采用Tunel法测算细胞凋亡率。用含0、30μg/mL白藜芦醇的培养基培养人眼结膜和皮肤病理性瘢痕成纤维细胞48 h,观察细胞的侵袭、迁移能力[侵袭率、迁移细胞数]。结果与0μg/mL白藜芦醇比较,含128、256μg/mL白藜芦醇的培养基培养的人眼结膜病理性瘢痕成纤维OD值均下降(P均<0.05);含64、128、256μg/mL白藜芦醇的培养基培养的皮肤病理性瘢痕成纤维OD值均下降(P均<0.05)。与0μg/mL白藜芦醇比较,含75μg/mL白藜芦醇的培养基培养的人眼结膜和皮肤病理性瘢痕成纤维细胞凋亡率升高(P均<0.05),含30μg/mL白藜芦醇的培养基培养的人眼结膜和皮肤病理性瘢痕成纤维细胞侵袭率、迁移数减少(P均<0.01)。结论含>64μg/mL白藜芦醇的培养基培养的人眼结膜和皮肤病理性瘢痕成纤维细胞活力下降,凋亡率升高,增殖、侵袭迁移能力降低。白藜芦醇可抑制人眼结膜和皮肤病理性瘢痕成纤维细胞增殖、侵袭、迁移,并促进其凋亡。Objective To observe the proliferation,apoptosis,invasion,and migration of human ocular conjunctival pterygium(ocular pathological scar)fibroblasts and human anterior thoracic skin pathological scars(skin-derived pathological scars)fibroblasts cultured in resveratrol-containing medium,in order to investigate the effects of resveratrol on the proliferation,apoptosis,invasion,and migration of human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts.Methods Primary human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts were isolated by tissue block adherent method.The cell viability(OD value)of human ocular and skin-derived pathological scar fibroblasts was detected by CCK-8 when the cells were cultured in media containing 0(control),1,2,4,8,16,32,64,128 and 256μg/mL resveratrol for 48 h.Human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts were cultured with 0 and 75μg/mL resveratrol for 8 h,and the apoptosis rate was measured by Tunel method.These two kinds fibroblasts were cultured in 0 and 30μg/mL resveratrol media for 48 h,and the migration and invasion abilities of the cells(the number of invasive migratory cells)were observed.Results The OD values of human ocular pathological scar fibroblasts cultured in media containing 128 and 256μg/mL resveratrol decreased(both P<0.05),and the OD values of skin-derived pathological scar fibroblasts cultured in media containing 64,128,and 256μg/mL resveratrol all decreased(all P<0.05),in comparison with those at 0μg/mL resveratrol.Compared with those at 0μg/mL resveratrol,the apoptosis rates of human ocular pathological scar fibroblasts and skin-derived pathological scar fi‐broblasts cultured in 75μg/mL resveratrol medium increased(all P<0.05),and the number of invasion and migration cells of human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts cultured in medium con‐taining 30μg/mL resveratrol became smaller(all P<0.01).C

关 键 词：白藜芦醇 成纤维细胞 病理性瘢痕 翼状胬肉 细胞增殖 细胞迁移 细胞凋亡 

分 类 号：R751[医药卫生—皮肤病学与性病学] R77[医药卫生—临床医学]