不同分子量透明质酸对牙周膜细胞成骨分化的影响  

The effects of hyaluronic acids with different weight on osteogenic differentiation of periodontal ligament cells

作　　者：金洁琪 光梦凯 JIN Jie-qi;GUANG Meng-kai(Department of Stomatology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院口腔科,北京100050 [2]中日友好医院口腔医学中心,北京100029

出　　处：《中日友好医院学报》2024年第5期283-286,F0003,共5页Journal of China-Japan Friendship Hospital

基　　金：中央高水平医院临床科研业务费资助(2024-NHL-HCRF-PYⅡ-01)。

摘　　要：目的:比较人牙周膜细胞(PDLCs)对4种不同分子量透明质酸(HA)的生物学反应。方法:将人牙周膜细胞(PDLCs)在培养基中单独培养21d(对照组)或添加成骨诱导(OS组)。其余4个实验组分别在OS培养基中添加不同分子量的HA:超低分子量透明质酸组(ULMWHA)、低分子量透明质酸组(LMWHA)、中分子量透明质酸组(MMWHA)和高分子量透明质酸组(HMWHA)。每天检查细胞形态。VonKossa染色和钙沉积法定性及定量分析不同分子量HA刺激下PDLCs的成骨分化和矿化。结果:随着时间的推移,单独使用OS或添加HA刺激的细胞显微镜下显示典型的成骨分化的外观。刺激21d后,各组均出现矿物沉积和钙沉积,其中LMWHA组表现出最显著的钙沉积。结论:透明质酸可以促进PDLCs的矿化,其中LMWHA作用较大。Objective:To compare the biological reactions of human periodontal ligament cells(PDLCs)to four distinct molecular weights of hyaluronic acid(HA)directly.Methods:PDLCs were cultured in medium alone for 21 days(control group)or were added with osteogenic stimulation(OS group)medium.The other 4 experimental groups were added with different MW HAs in OS medium:ultra-low molecular weight hyaluronic acid group(ULMW HA),low molecular weight hyaluronic acid group(LMW HA),medium molecular weight hyaluronic acid group(MMW HA)and high molecular weight hyaluronic acid group(HMW HA).The cell morphology was checked daily.The osteoblast differentiation and mineralization of PDLCs stimulated by different MW HAs were analyzed qualitatively and quantitatively by Von Kossa staining and calcium deposition.Results:Over time,cells stimulated with OS alone or with the addition of HAs showed the microscopic appearance of all typical osteogenic differentiation.After 21 days of stimulation,mineral and calcium deposits appeared in all groups except control group,and the LMW HA group showed the most significant calcium deposition.Conclusion:HA can promote the mineralization of PDLCs,in which LMW HA being the more effective.

关 键 词：牙周膜细胞 骨形成 成骨分化 透明质酸 分子量 

分 类 号：R34[医药卫生—基础医学]