基于饲养层细胞培养鸡小肠上皮细胞及紫锥菊提取物对其增殖的影响研究  

The Cell Culture of Chicken Intestinal Epithelial Cell Based on Feeder Layer Cells and Effects of Echinacea Extract on cell Proliferation

作　　者：吉艺宽 李美娣 JI Yikuan;LI Meidi(Guangdong Meizhou Vocational and Technical College,Meizhou Engineering Research Center of Traditional Veterinary Medicine,Meizhou Guangdong 514011)

机构地区：[1]广东梅州职业技术学院/梅州市中兽药工程技术研究中心,广东梅州514011

出　　处：《广东畜牧兽医科技》2024年第5期37-40,共4页Guangdong Journal of Animal and Veterinary Science

基　　金：广东省普通高校特色创新项目(自然科学)(2023KTSCX344);2022年度梅州市社会发展科技计划项目(2022B125)。

摘　　要：该研究为了探究鸡肠道成纤维细胞作为饲养层细胞培养鸡肠道上皮细胞(IEC)的可能性及紫锥菊提取物对IEC的增殖影响。试验首先通过IV型胶原酶消化法得到鸡肠道成纤维细胞,并采用丝裂霉素C将其制备成饲养层细胞与分离的鸡肠道上皮细胞共培养。通过细胞免疫荧光鉴定鸡IEC角蛋白的表达、细胞计数试剂(CCK-8)法检测其细胞增殖能力及紫锥菊提取物对其增殖的影响。结果表明:分离的鸡肠道上皮细胞表达角蛋白,与饲养层细胞共培养增殖能力良好,体外存活达14 d;此外31.25-2000μg/mL的紫锥菊提取物能有效促进受损的鸡肠道上皮细胞增殖,其中浓度为500μg/mL效果最为显著(P<0.01)。因此,鸡肠道成纤维细胞可以作为饲养层细胞培养鸡肠道上皮细胞,且紫锥菊提取物对其增殖有促进作用。In order to explore the possibility of using chicken intestinal fibroblasts as feeder cells to cultivate chicken intestinal epithelial cells and the proliferation effect of Echinacea extract on IEC.In this experiment,the chicken intestinal fibroblasts were obtained by digestion by the type IV collagenase,and were prepared into feeder cells using mitomycin C and co-cultured with isolated chicken intestinal epithelial cells.The expression of chicken IEC Cytokeratin protein was determined by cellular immunofluorescence,and the proliferation ability of chicken cells and the effect of echinacea extract on the proliferation were detected by CCK-8 method.The isolated chicken intestinal epithelial cells expressed Cytokeratin protein,had good proliferation ability in co-culture with feeder cells,and survived for 14 days in vitro.In addition,the damaged chicken intestinal epithelial cells could promoted effectively by echinacea extract of 31.25-2000μg/mL The results show that chicken intestinal fibroblasts can be used as feeder cells to culture chicken intestinal epithelial cells,and echinacea extract can promote their proliferation.This experiment provides a new way for IEC culture in vitro,and also provides a reference for the study of echinacea extract on intestinal mucosal immunity.

关 键 词：鸡肠道上皮细胞 饲养层细胞 紫锥菊 

分 类 号：S816.7[农业科学—饲料科学]