鸡与小鼠耳蜗损伤模型的构建及毛细胞再生的比较  

Construction of Cochlear Injury Models and Comparison of Hair Cell Regeneration of Chicks and Mice

作　　者：张信文 杨思远[1,2] 白焕菊 江凌玲 翁梦露 陈开明 曾少举 ZHANG Xinwen;YANG Siyuan;BAI Huanju;JIANG Linging;WENG Mengu;CHEN Kaiming;ZENG Shaoju(College of Pharmacy,Hainan Vocational University of Science and Technology,Haikou 571126,China;College of Life Sciences,Beijing Normal University,Beijing 100875,China;College of Life Science,Hainan Normal University,Haikou 571158,China;The High School Affiliated to Renmin University of China,Beijing 100080,China;Beijing Tiantan Hospital,Capital Medical University,Beijing 100070,China;Beipo Junior High School,Wanning Hainan 571526,China)

机构地区：[1]海南科技职业大学医药学院,海口571126 [2]北京师范大学生命科学学院,北京100875 [3]海南师范大学生命科学学院,海口571158 [4]中国人民大学附属中学,北京100080 [5]首都医科大学附属北京天坛医院,北京100070 [6]海南省万宁市北坡初级中学,海南万宁571526

出　　处：《海南热带海洋学院学报》2024年第5期128-135,共8页Journal of Hainan Tropical Ocean University

基　　金：国家自然科学基金项目(31860269);海南省自然科学基金项目(319QN238)。

摘　　要：氨基糖苷类抗生素在治疗疾病过程中产生的副作用导致众多患者出现听力损失,这类抗生素更易于引发孵化后期鸡胚、新生雏鸡、胚胎期及初生小鼠,以及人类新生儿和幼儿耳蜗毛细胞的损伤。通过构建鸡胚、雏鸡和小鼠耳蜗损伤模型,并评估毛细胞在遭受损伤后的再生与修复潜力,结果显示,鸡与小鼠耳蜗毛细胞在损伤与再生过程中存在显著差异:小鼠毛细胞更容易受到损伤,而且损伤后不具备再生能力;鸡毛细胞在受损后展现出较强的再生潜能,能够自我修复并恢复听力功能。构建耳蜗损伤的动物模型可探索如何将鸡特有的毛细胞再生机制移植或模拟应用于小鼠模型中,为进一步研究如何预防和修复人类婴幼儿使用抗生素导致的内耳毛细胞损伤提供一定的基础资料。The side effects of aminoglycoside antibiotics in treating diseases lead to hearing loss in many patients.These antibiotics are more likely to trigger damage to cochlear hair cells in late‐stage chicken embryos,newborn chicks,embryonic and newborn mice,and human newborns and young children.By constructing models of cochlear injury in chicken embryos,chicks,and mice,and evaluating the regeneration and repair potential of hair cells after injury,the results showed significant differences in hair cell injury and regeneration processes between chicks and mice.Mouse hair cells are more susceptible to damage and have no regenerative capacity after injury.In contrast,chicken hair cells show strong regen‐eration potential and can self‐repair and restore their auditory function after injury.The construction of an animal model of cochlear injury can explore how to transplant or simulate the unique hair cell regeneration mechanism of chickens into the mouse model and provide some basic information for further research on how to prevent and repair inner ear hair cell damage caused by antibiotic use in human infants and children.

关 键 词：氨基糖苷类抗生素 耳蜗损伤模型 再生 鸡 小鼠 

分 类 号：R764[医药卫生—耳鼻咽喉科] Q95-3[医药卫生—临床医学]