‘玛瑙红’樱桃胚败育果实和正常果实比较转录组分析  

Comparative Transcriptome Analysis between Embryo Ebortion and Normal Fruit of the Prunus pseudoceresrs'Manaohong'

作　　者：宋莎 江旭升[2] 金吉林[1] 张绿萍[1] 张敏[1] SONG Sha;JIANG Xusheng;JIN Jilin;ZHANG Lüping;ZHANG Min(Institute of Fruit Science,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;Guiyang Agricultural Test Center,Guiyang 550018,China)

机构地区：[1]贵州省农业科学院果树科学研究所,贵阳550006 [2]贵阳市农业试验中心,贵阳550018

出　　处：《种子》2024年第10期43-48,53,共7页Seed

基　　金：贵州省科技计划项目(黔科合基础-ZK[2022]一般233)。

摘　　要：为探索‘玛瑙红’樱桃在胚败育情况下果实仍正常发育的分子机理,本研究采用Illumina高通量测序技术,对不同发育时期的‘玛瑙红’樱桃胚败育果实和正常果实样品进行转录组测序及相关分析。结果共获得98.87%的Clean Reads,86.24%的Mapped Reads,Q30>94.21%;共筛选出43673个差异表达基因,其中所有样品组合共有的差异表达基因为8213个,大多分布在FPKM 0~0.01区间。富集分析显示,注释到GO的差异表达基因参与分子功能、细胞组分、生物过程等,其中参与生物过程的最多;注释到KEGG通路的差异表达基因分别参与环境信息处理、生物体系统、遗传信息处理、细胞过程和新陈代谢五大通路的18个分支,大多注释到新陈代谢通路;预测为转录因子的差异表达基因共2002个,共注释到53个转录因子家族,其中ERF家族最多。研究表明,‘玛瑙红’樱桃胚败育果实与正常发育果实在膨大期基因表达差异较大,果实转色期至果实成熟基因表达差异逐渐变小,这可能是胚败育果实正常成熟的原因。In order to explore the molecular mechanism of normal development of Prunus pseudoceresrs'Manaohong'despite embryo abortion,this study used Illumina high-throughput sequencing technology to perform transcriptome sequencing and correlation analysis on samples of Prunus pseudoceresrs'Manaohong'embryo abortion and normal fruit at different developmental stages.Results showed that 98.87%of Clean Reads and 86.24%of Mapped Reads were obtained,with Q30>94.21%.A total of 43673 differentially expressed genes were screened,among which 8213 differentially expressed genes were common to all sample combinations,mostly distributed in the FPKM from 0 to 0.01 range.Enrichment analysis showed that the differentially expressed genes annotated to GO participated in molecular function,cell components,biological processes,and so on,and most of them participated in biological processes.The differentially expressed genes annotated to KEGG pathway were involved in 18 branches of five pathways:environmental information processing,biological system,genetic information processing,cell process and metabolism,and most of them were annotated to metabolic pathway.A total of 2002 differentially expressed genes predicted to be transcription factors were annotated to 53 transcription factor families,among which ERF family was the largest.Study showed that the gene expression difference between Prunus pseudoceresrs'Manaohong'embryo aborted fruit and normal development fruit was great in expansion stage,and the gene expression difference gradually decreased from fruit color transformation stage to fruit maturity,which could be the cause of normal maturation of embryo aborted fruit.

关 键 词：‘玛瑙红’樱桃 胚败育 差异表达基因 转录组测序 

分 类 号：S662.5[农业科学—果树学]