基于TTC染色的水稻种子活力定量分析  

Quantitative Analysis of Rice Seed Viability Based on TTC Staining

作　　者：乔菊香 冯明春 杨晓洪[1,2] Hoàng Minh Tú 王世敏 刘艳芳 QIAO Juxiang;FENG Mingchun;YANG Xiaohong;Hoang Minh Tú;WANG Shimin;LIU Yanfang(Quality Standards and Testing Technology Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;Kunming Sub-Center for Test of New Varieties of Plants,Ministry of Agricultural and Rural Affairs,Kunming 650205,China;School of Agriculture and Life Sciences,Zhaotong University,Zhaotong Yunnan 657000,China;Vietnam National Testing Center for Plant Seeds and Agricultural Products,Hanoi Vietnam 11611)

机构地区：[1]云南省农业科学院质量标准与检测技术研究所,昆明650205 [2]农业农村部植物新品种测试(昆明)分中心,昆明650205 [3]昭通学院农学与生命科学学院,云南昭通657000 [4]越南国家植物种子和农产品检测中心,越南河内11611

出　　处：《种子》2024年第10期135-141,共7页Seed

基　　金：昆明市科技局“中-越-缅水稻种子质量检测科技合作基地项目(GHJD-2022012)”;云南省科技厅农业联合专项-面上项目计划“不同类型和地理来源的水稻种子发芽力特征及其分子机理研究项目”(202401BD070001-084);云南省科技厅“2022年‘智汇云南’计划-青年科学家、青年企业家Hoàng Minh Tú项目(202303AM140011)”;云南省科技厅“2022年云南省国际科技特派员(个人)认定工作项目(202303AK140026)”;科技部“生物育成品种DUS测试工具装备研发项目(2023ZD0406106)”资助。

摘　　要：为了揭示种子活力与TTC染色之间的定量关系,本研究以水稻种子CY-150为试验材料,经人工加速老化处理和TTC染色后,利用体视镜获取种子胚部纵切面的RGB图像,并用农业农村部植物新品种测试(昆明)分中心建立的图像分析软件分析图像的CIELab值,计算单位面积染色强度。结果表明,发芽率、TTC染色率和活力指数之间呈显著正相关。其中,发芽率和活力指数、发芽率和染色率、染色率和活力指数之间的相关系数分别为0.9976、0.9718和0.9631。此外,CIELab值与发芽率、染色率和活力指数之间显著相关,其中,L^(*)和a^(*)分别呈负相关和正相关,相关系数分别为-0.9749、-0.9761、-0.9721、0.9737、0.9915和0.9678。利用种子活力指数与CIELab值建立了种子活力方程。通过随机选取4个水稻品种对方程进行验证,结果显示,4个品种的活力指数真实值略高于预测值。研究表明,TTC染色不仅可用于水稻种子活力快速定性分析,而且通过种胚RGB图像可以实现种子活力的定量分析,为进一步普及水稻种子活力快速检测提供参考。In order to reveal the quantitative relationship between seed vitality and TTC staining,this study took rice(Oryza sativa L.)seed CY-150 as the experimental material.After artificial accelerated aging treatment and TTC staining,stereoscopic RGB images of the longitudinal section of seed embryo were obtained,and the CIELab values of the images were analyzed by the image analysis software established by the Plant New Variety Testing(Kunming)Sub-center of the Ministry of Agriculture and Rural Affairs,and calculating the staining intensity per unit.The results showed that there was a significant positive correlation between germination rate,TTC staining rate and vigor index.Among them,the correlations between germination rate and vitality index,germination rate and staining rate,staining rate and vitality index were 0.9976,0.9718 and 0.9631.In addition,CIELab value was significantly correlated with germination rate,staining rate and vitality index,in which L^(*)and a^(*)were negatively correlated and positively correlated,and Pearson correlation coefficients were-0.9749,-0.9761,-0.9721,0.9737,0.9915 and 0.9678,respectively.At the same time,the seed vigor equation was established using seed vigor index and CIELab value.Four rice varieties were randomly selected to verify the equation,and the results showed that the real values of the vigour index of the four varieties were slightly higher than the predicted values.The results showed that TTC staining could not only be used for rapid qualitative analysis of rice seed viability,but also could be used for quantitative analysis of seed viability through seed embryo RGB images,which provided a reference for further popularization of rapid detection of rice seed viability.

关 键 词：水稻 种子活力 TTC染色 定量分析 

分 类 号：S511[农业科学—作物学]