伏牛山野生黄精组培快繁体系建立及组培苗质量评估  

Establishment of a Tissue Culture System with Quality Assessment for Wild Polygonatum sibiricum in Funiu Mountain

作　　者：董薇[1] 余永亮[1] 许兰杰[1] 杨红旗[1] 安素妨 杨青 苏小雨 梁慧珍[1] DONG Wei;YU Yongliang;XU Lanjie;YANG Hongqi;AN Sufang;YANG Qing;SU Xiaoyu;LIANG Huizhen(Institute of Chinese Herbal Medicine,He'nan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院中药材研究所,郑州450002

出　　处：《种子》2024年第10期142-147,共6页Seed

基　　金：财政部和农业农村部:国家现代农业产业技术体系(CARS-21);河南省重大科技专项(221100310400);河南省重点研发专项(231111110800);中央本级重大增减支项目“名贵中药资源可持续利用能力建设项目”(2060302);河南省农科院自主创新专项基金(2023ZC083);河南省科技攻关项目(232102110262,242102310531,242102110248);河南省农业科学院优秀青年科技基金(2024YQ16)。

摘　　要：以伏牛山野生黄精的种子为试验材料,建立其组培快繁体系并对组培苗质量进行评估。结果表明,伏牛山野生黄精种子萌发最佳培养基为1/2 MS+0.5 mg/L ABA,丛生芽诱导最佳培养基为MS+4.0 mg/L 6-BA+0.2 mg/L NAA+0.5 mg/L GA3,丛生芽增殖最佳培养基为MS+3 mg/L 6-BA+0.1 mg/L TDZ+0.5 mg/L 2,4-D+0.4 mg/L NAA,生根最佳培养基为1/2 MS+0.5 mg/L NAA+0.2 mg/L IBA;组培苗分级标准为Ⅰ级苗:株高≥4.2 cm、叶片≥5个,Ⅱ级苗:3 cm<株高<4.2 cm、2<叶片数<5,Ⅲ级苗株高≤3 cm、叶片数≤2个,移栽后成活率为88%。The tissue culture rapid propagation system was established and the quality of the tissue culture seedlings was evaluated with the wild Polygonatum sibiricum seeds from Funiu Mountain as the research material.The results showed that the best medium for seed germination of Polygonatum sibiricum from Funiu Mountain was 1/2 MS+0.5 mg/L ABA,the best medium for cluster bud induction was MS+4.0 mg/L 6-BA+0.2 mg/L NAA+0.5 mg/L GA 3,and the best medium for cluster bud proliferation was MS+3 mg/L 6-BA+0.1 mg/L TDZ+0.5 mg/L 2,4-D+0.4 mg/L NAA,the best rooting medium was 1/2 MS+0.5 mg/L NAA+0.2 mg/L IBA.The classification standard of tissue culture seedlings was GradeⅠ:plant height≥4.2 cm,leaves≥5,GradeⅡ:3 cm<plant height<4.2 cm,2<number of leaves<5,GradeⅢ:plant height≤3 cm,number of leaves≤2,the survival rate after transplantation was 88%.

关 键 词：黄精 种子 组培 组培苗质量 

分 类 号：S567.239[农业科学—中草药栽培]