黄梁木叶精油对柱花草和水稻秸秆发酵品质及细菌群落的影响  

Effect of Neolamarckia cadamba Leaf Essential Oil on Fermentation Quality and Bacterial Community of Stylo and Rice Straw Silage

作　　者：周雨歆 杨丹 杨伟涛 陈丹丹 陈晓阳 张庆 ZHOU Yu-xin;YANG Dan;YANG Wei-tao;CHEN Dan-dan;CHEN Xiao-yang;ZHANG Qing(College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou,Guangdong Province 510642,China)

机构地区：[1]华南农业大学林学与风景园林学院/广东木本饲料工程技术研究中心/广东省森林植物种质创新与利用重点实验室,广东广州510642

出　　处：《草地学报》2024年第10期3326-3334,共9页Acta Agrestia Sinica

基　　金：广东长隆慈善基金会(CLPF2021007Z);国家自然科学基金项目(31771345);广东省林业科技创新项目(2019KJCX001)资助。

摘　　要：为探究黄梁木(Neolamarckia cadamba)叶精油分别对柱花草(Stylosanthes guianensis L.)和水稻(Oryza sativa L.)秸秆青贮发酵品质和细菌群落结构的影响,本试验基于新鲜柱花草和水稻秸秆重量的0.5%和1%添加黄梁木叶精油(NCEO)进行青贮混合处理,每个处理3组重复,装入聚乙烯密封袋并用真空封口机密封,青贮30 d后测定样品的青贮发酵品质指标,分析细菌群落结构。结果表明:添加黄梁木叶精油显著降低了柱花草和水稻秸秆青贮饲料中pH值、氨态氮含量、乳酸菌和大肠菌群数量(P<0.05),显著提高了水稻秸秆青贮饲料的乳酸和真蛋白含量(P<0.05)。乳酸菌在水稻秸秆青贮饲料中占优势地位,且数量随黄梁木叶精油添加量的增加而升高。黄梁木叶精油的添加也增加了异根瘤菌属、甲基杆菌属和黄单胞杆菌属的相对丰度。综上所述,添加1%黄梁木叶精油可有效改善柱花草和水稻秸秆青贮发酵品质。The objective of this experiment was to explore the effect of Neolamarkia cadamba leaf essential oil(NCEO)on the fermentation quality and bacterial community of stylo(Stylosanthes guianensis L.)and rice(Oryza sativa L.)straw silage.Based on the weight of fresh stylo and rice straw,adding the 0.5%and 1%NCEO into materials for 30 days ensiling.Vacuumed and sealed after three replicates of each treatment were packed into plastic bags(20 cm×30 cm).After 30 days of ensiling,the chemical composition and silage indexes of the samples were evaluated statistically.The results of fermentation parameter,protein fraction and bacterial community indicated that NCEO addition decreased(P<0.05)pH value,ammonia-N content,and numbers of lactic acid bacteria and coliform bacteria both in stylo and rice straw silage.And lactic acid content and true protein proportion of rice straw silage increased(P<0.05)with NCEO addition.Lactobacillus was the most dominant genus and increased with NCEO addition in rice straw silage.The NCEO addition also increased the relative abundance of Allorhizobium,Methylobacterium and Xanthomonas.In conclusion,the 1%NCEO addition was an effective way to improve the fermentation of stylo and rice straw silage.

关 键 词：黄梁木叶精油 青贮品质 细菌群落 柱花草 水稻秸秆 

分 类 号：S816.53[农业科学—饲料科学]