脂代谢紊乱破坏线粒体功能导致大鼠动脉粥样硬化形成  

Lipid metabolism disorders destroy mitochondrial function and lead to atherosclerosis in rats

作　　者：宋子凯[1] 李忠乐 赵亚明 叶琳[4] SONG Zikai;LI Zhongle;ZHAO Yaming;YE Lin(Department of Cardiology,the First Hospital of Jilin University,Changchun 130021,China;The Third Affiliated Hospital of Jinzhou Medical University,Jinzhou,Liaoning 121001,China;School of Basic Medicine,Jilin University,Changchun,130021,China;Department of Occupational Health and Environmental Health,School of Public Health,Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学第一医院心血管内科,吉林长春130021 [2]锦州医科大学附属第三医院,辽宁锦州121001 [3]吉林大学基础医学院,吉林长春130021 [4]吉林大学公共卫生学院劳动卫生与环境卫生教研室,吉林长春130021

出　　处：《中国实验诊断学》2024年第10期1218-1224,共7页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技厅项目(20200201523JC,20200403113SF)。

摘　　要：目的通过大鼠高脂饮食模型了解脂代谢相关基因mRNA表达水平和线粒体功能在主动脉粥样硬化形成中的作用。方法高脂饮食联合维生素D3建立大鼠动脉粥样硬化模型,检测血清中血脂指标以及脂质代谢基因和线粒体合成和分解基因mRNA表达水平。结果模型组主动脉和颈动脉动脉粥样硬化形成明显,并且随着时间的延长病变加重。与对照组比较,模型组Lp(a)、TC、TG和LDL-C水平明显高于对照组(P<0.05),apoB100和HDL-C差异无统计学意义(P>0.05)。另外,模型组主动脉组织脂代谢相关基因SREBF2、LDL、HMGCR、FDFT1、HMGCS 1、LPL和DHCR24基因以及线粒体融合和分解基因MFN2、Drp1 mRNA表达水平明显增高(P<0.05),ABCA和SQLE mRNA表达水平无明显变化(P>0.05)。结论高脂饮食导致血脂增高是动脉粥样硬化形成的关键。主动脉病变组织脂质代谢基因mRNA表达水平明显增高,线粒体融合和分解明显增加。Objective To investigate the role of mRNA expression level of lipid metabolism related genes and mitochondrial function in the formation of aortic atherosclerosis through a high-fat diet model in rats.Methods The atherosclerotic model of rats was established by high-fat diet combined with vitamin D3.Serum lipid indexes and mRNA expression levels of lipid metabolism genes and mitochondrial synthesis and decomposition genes were detected.Results Atherosclerosis of aorta and carotid artery in the model group was obvious,and the lesion aggravated with time.Compared with the control group,the levels of Lp(a),TC,TG,and LDL-C in the model group were significantly higher than those in the control group(P<0.05),while there was no statistical difference in apoB100and HDL-C(P>0.05).In addition,the mRNA expression levels of lipid metabolism related genes SREBF2,LDL,HMGCR,FDFT1,HMGCS 1,LPL,and DHCR24,as well as mitochondrial synthesis and decomposition genes MFN2and Drp1,were significantly increased in the model group(P<0.05),while there was no significant change in the mRNA expression levels of ABCA and SQLE(P>0.05).Conclusion The key to atherosclerosis is the increase of blood lipid caused by high-fat diet.The mRNA expression level of lipid metabolism genes in aortic lesion tissues is significantly increased,and mitochondrial synthesis and decomposition are significantly increased.

关 键 词：脂代谢紊乱 动脉粥样硬化 线粒体 

分 类 号：R543.5[医药卫生—心血管疾病]