十堰市白菜黑斑病病原真菌的分离与鉴定  

Separation and identification of pathogenic fungi causing Chinese cabbage black leaf spot disease in Shiyan City

作　　者：石宪铭 何亚慧 胡燕萍 吴飞扬 彭晓璇 张勇洪 游磊 温毅华 SHI Xian-ming;HE Ya-hui;HU Yan-ping;WU Fei-yang;PENG Xiao-xuan;ZHANG Yong-hong;YOU Lei;WEN Yi-hua(School of Basic Medical Sciences,Hubei University of Medicine,Shiyan 442000,Hubei,China;Biomedical Research Institute/Hubei Key Laboratory and Engineering Technology Center of Wudang Local Chinese Medicine Research,Hubei University of Medicine,Shiyan 442000,Hubei,China;Shiyan Academy of Agricultural Sciences,Shiyan 442000,Hubei,China;Shiyan Vegetable Science Research Institute,Shiyan 442000,Hubei,China)

机构地区：[1]湖北医药学院基础医学院,湖北十堰442000 [2]湖北医药学院生物医药研究院/武当特色中药研究湖北省重点实验室与工程技术中心,湖北十堰442000 [3]十堰市农业科学院,湖北十堰442000 [4]十堰市蔬菜科学研究所,湖北十堰442000

出　　处：《湖北农业科学》2024年第10期59-63,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(32200680);湖北省教育厅项目(JYT2021009)。

摘　　要：对湖北省十堰市当地白菜(Brassica pekinensis)品种的黑斑病进行病原真菌鉴定和病害分析,并提出相应的预防措施。采用组织分离法从病叶中分离得到病原真菌,并利用rDNA ITS和Alt-α1基因序列比对进行分析鉴定。结果表明,发现1个新的链格孢属(Alternaria sp.)真菌,推测其是导致十堰市当地白菜黑斑病的致病真菌。此外,确认ITS序列和Alt-α1基因序列的联合分析,能够区分3种常见的链格孢属真菌Alternaria brassicicola、Alternaria japonica、Alternaria brassicae。The pathogenic fungi of the black spot of Brassica pekinensis in Shiyan City,Hubei Province were identified and analyzed,and the corresponding preventive measures were proposed.The pathogenic fungi were isolated from diseased leaves by tissue separation and identified by rDNA ITS and Alt-α1 gene sequence alignment.The results showed that a new Alternaria sp.fungus was found,which was presumed to be the pathogenic fungus causing the local cabbage black spot disease in Shiyan City.In addition,it was confirmed that the joint analysis of ITS sequence and Alt-α1 gene sequence could distinguish three common Alternaria fungi:Alternaria Brassicola,Alternaria japonica and Alternaria brassicae.

关 键 词：白菜(Brassica pekinensis) rDNA ITS 黑斑病 病原真菌 分离与鉴定 湖北省十堰市 

分 类 号：S436.341[农业科学—农业昆虫与害虫防治]