拟南芥中异源过表达CsLOX6和CsHPL2基因提高植株耐盐性和耐旱性  

Heterologous overexpression of CsLOX6 and CsHPL2 genes in Arabidopsis thaliana enhances plant salt and drought tolerance

作　　者：王聪 李强[1] 柴琳 王恒 余宏军[1] 蒋卫杰[1] WANG Cong;LI Qiang;CHAI Lin;WANG Heng;YU Hong-jun;JIANG Wei-jie(Institute of Vegetables and Flowers/State Key Laboratory of Vegetable Biobreeding,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所/蔬菜生物育种全国重点实验室,北京100081

出　　处：《湖北农业科学》2024年第10期165-175,共11页Hubei Agricultural Sciences

基　　金：国家大宗蔬菜产业技术体系项目(CARS-23-B07);国家自然科学基金项目(31672203);国家重点研发计划支持项目(2023YFD2300703);现代农业产业技术体系北京市创新团队项目(BAIC-2024)。

摘　　要：探究CsLOX6基因和CsHPL2基因对拟南芥(Arabidopsis thaliana)生长以及对干旱和盐胁迫抗性的影响,以野生型拟南芥(WT)、转基因型拟南芥(OE-CsLOX6、OE-CsHPL2)、突变体型拟南芥(TB-Atlox5)为材料,通过表型观察、转录组和代谢组学分析等方法评估4种拟南芥的耐旱性、耐盐性。结果表明,OE-CsLOX6的地上部鲜重和根长均显著高于其他基因型植株,地下部鲜重明显高于其他基因型植株;OE-CsHPL2的地上部鲜重、地下部鲜重和根长明显低于其他基因型植株。OE-CsLOX6、OE-CsHPL2和WT的代谢物含量存在较大差异,OE-CsLOX6和WT有64个差异代谢物,其中有6个上调,58个下调;OE-CsHPL2和WT有63个差异代谢物,其中有9个上调,54个下调。过表达CsLOX6基因和CsHPL2基因可以提高拟南芥对盐胁迫和干旱胁迫的抗性。在盐胁迫和干旱胁迫下,OE-CsLOX6、OE-CsHPL2的丙二醛含量要显著低于野生型植株,膜质过氧化程度低,SOD、POD和CAT活性要明显高于野生型,提高了植株的抗氧化能力,叶片长势明显优于野生型植株。To explore the effects of CsLOX6 gene and CsHPL2 gene on the growth of Arabidopsis thaliana and its resistance to drought and salt stress,wild-type Arabidopsis thaliana(WT),transgenic Arabidopsis thaliana(OE-CsLOX6,OE-CsHPL2),and mutant Arabidopsis thaliana(TB-Atlox5)were used as materials,and the drought and salt tolerance of the four Arabidopsis thaliana species were evaluated through phenotype observation,transcriptome,and metabolomics analysis.The results showed that the aboveground fresh weight and root length of OE-CsLOX6 were significantly higher than those of other genotypes,and the underground fresh weight was significantly higher than that of other genotypes;the aboveground fresh weight,underground fresh weight,and root length of OE-CsHPL2 plants were significantly lower than those of other genotypes.There were significant differences in the metabolite content between OE-CsLOX6,OE-CsHPL2,and WT.OE-CsLOX6 and WT had 64 differential metabolites,of which 6 were upregulated and 58 were downregulated;OE-CsHPL2 and WT had 63 differential metabolites,of which 9 were upregulated and 54 were downregulated.Overexpression of CsLOX6 gene and CsHPL2 gene could enhance the resistance of Arabidopsis thaliana to salt stress and drought stress.Under salt stress and drought stress,compared with wild-type plants,the malondialdehyde content of OE-CsLOX6 and OE-CsHPL2 was significantly lower,and the degree of membrane peroxidation was lower,the activities of SOD,POD,and CAT enzymes were significantly higher than those of the wild type,which improved the antioxidant capacity of the plants and resulted in significantly better leaf growth compared to the wild type plants.

关 键 词：拟南芥(Arabidopsis thaliana) 异源过表达 CsLOX6基因 CsHPL2基因 耐盐性 耐旱性 胁迫 

分 类 号：S642.2[农业科学—蔬菜学]