潜阳育阴方联合缬沙坦对自发性高血压大鼠肠道菌群失调的改善作用  

Ameliorating Effect of Qianyang Yuyin Decoction and Valsartan on Gut Microbiota Dysbiosis in Spontaneously Hypertensive Rats

作　　者：朱其辉 周镯 曹园 ZHU Qihui;ZHOU Zhuo;CAO Yuan(The Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Privince Hospital of Chinese Medicine,Department of Pharmaceutical Preparation,Nanjing 210029,China;The Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Privince Hospital of Chinese Medicine,Department of Pharmacy,Nanjing 210029,China)

机构地区：[1]南京中医药大学附属医院/江苏省中医院制剂部,江苏南京210029 [2]南京中医药大学附属医院/江苏省中医院药学部,江苏南京210029

出　　处：《现代中药研究与实践》2024年第4期37-42,共6页Research and Practice on Chinese Medicines

基　　金：江苏省中医药局科技项目(JD201813);江苏高校优势学科建设工程资助项目(ZYX03KF074);江苏省研究生科研与实践创新计划(SJCX21_0670)。

摘　　要：目的 研究潜阳育阴方联合缬沙坦对自发性高血压(SHR)大鼠肠道菌群失调的改善作用。方法 32只12周龄雄性SHR大鼠随机分成模型组(MG)、缬沙坦组(XG)、潜阳育阴组(ZG)和中西药联用组(ZXG),以12周龄雄性Wistar-Kyoto(WKY)大鼠为正常组(CG),每组8只。连续灌胃8周后,提取大鼠新鲜粪便样本,进行16S rRNA基因测序,并于给药前及给药后第8周清醒时检测大鼠的收缩压(SBP)与舒张压(DBP)。结果 ZXG组降压效果明显强于ZG组。菌群结构分析显示在属水平上,MG组Coprobacillus、Lactococcus、Phascolarctobacterium、Eubacterium、Elusimicrobium和Enterococcus等菌属显著下调;而ZG组能够逆转SHR大鼠Coprobacillus和Eubacterium水平,ZXG组逆转Phascolarctobacterium、Enterococcus、Eubacterium和Lactococcus水平。PICRUSt功能预测表明主要调控糖代谢和能量代谢功能;中西药联用主要调控糖代谢、能量代谢和PPAR信号通路。结论 潜阳育阴方联合缬沙坦能够改善SHR大鼠肠道菌群失调,可为二者联用治疗原发性高血压提供理论依据。Objective To explore the regulatory effect of Qianyang Yuyin decoction combined with valsartan on the gut microbiota dysbiosis in spontaneously hypertensive rats(SHRs).Methods 32 male SHR rats of 12 weeks old were randomly divided into modelgroup(MG),valsartan group(XG),Qianyang Yuyingroup(ZG)and Qianyang Yuyin combined valsartan group(ZXG),as well as 8 male Wistar-Kyoto rats(WKYs)of the same age were as control group(CG).The systolic blood pressure(SBP)and the diastolic blood pressure(DBP)of rats were monitored by noninvasive blood pressure measurement of tail artery before and after 8 weeks of treatment,respectively.After 8 weeks of administration,the fecal DNA was extracted and 16S rRNA gene was sequenced and the results were analyzed.The systolic blood pressure(SBP)and the diastolic blood pressure(DBP)of rats were monitored by noninvasive blood pressure measurement of tail artery before and after 8 weeks of treatment,respectively.Results The anti-hypertensive potency of herb-drug combination intervention was much better than Qianyang Yuyin decoction.The relative abundances of Coprobacillus,Lactococcus,Phascolarctobacterium,Eubacterium,Elusimicrobium and Enterococcus were reduced in MG group,however,the relative abundances of Coprobacillus and Eubacterium were reversed after Qianyang Yuyin decoction treatment;and those of Phascolarctobacterium,Enterococcus,Eubacterium,Lactococcus were reversed after herb-drug combination treatment.PICRUSt function prediction showed that Qianyang Yuyin decoction combined with valsartan could restore the disordered gut microbiota composition and metabolism.Conclusion Qianyang Yuyin decoction combined with valsartan can exert anti-hypertensive effect by restoring gut microbiota dysbiosis in SHRs,which provide theoretical basis for herb-drug combination.

关 键 词：16S rRNA测序 潜阳育阴方 缬沙坦 中西药联用 自发性高血压大鼠 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]