杭蓟提取物对小鼠慢性酒精性肝损伤的保护作用及机制研究  

Protective Effect and Mechanism of Cirsium tianmushanicum Shih Extract on Chronic Alcoholic Liver Injury in Mice

作　　者：王笑雪 王宇航[1,3] 郝巨辉 叶亦卿[4] 刘勇 李卫东 WANG Xiaoxue;WANG Yuhang;HAO Juhui;YE Yiqing;LIU Yong;LI Weidong(School of Chinese Materia Medica,Bejjing University of Chinese Medicine,Beijing 102488,China;Collaborative Innovation Center of Aerospace and Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China;Engincering Research Center of Good Agricultural Practice for Chinese Crude Drugs,Ministry of Education,Beiing 102488,China;Chaoqing(Ningbo)Biotechnology Co.,LTD.,Ningbo 315602,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]北京中医药大学航空航天中医药协同创新中心,北京102488 [3]中药材规范化生产教育部工程研究中心,北京102488 [4]超卿(宁波)生物科技有限公司,浙江宁波315602

出　　处：《现代中药研究与实践》2024年第4期43-49,共7页Research and Practice on Chinese Medicines

基　　金：北京中医药大学科研A级横向科研发展基金(90020272220003)。

摘　　要：目的 研究杭蓟提取物对小鼠慢性酒精性肝损伤的保护作用及机制。方法 36只C57BL/6小鼠随机分为正常组、模型组、联苯双酯组与杭蓟提取物低、中、高剂量组,每组6只。正常组以生理盐水灌胃,其余各组用50%乙醇灌胃,1 h后正常组和模型组灌胃生理盐水,给药组按相应剂量灌胃。30 d后测定小鼠肝脏指数、血清与肝脏生化指标,观察肝脏、小肠组织病理形态及肝脏细胞凋亡情况,Western Blotting检测Bax/Bcl-2/Csapase-3信号通路蛋白表达。结果 与模型组相比,给药组小鼠肝脏指数降低、ALT、AST活性与MDA、TG含量降低(P<0.01),GSH水平升高(P<0.01),肝脏与小肠组织形态明显改善,Bax、Cyt-C、Cleaved Caspase-3 蛋白表达降低(P<0.01),Bcl-2蛋白表达升高(P<0.01),各组Caspase-3蛋白水平,差异无统计学意义(P>0.05)。结论 杭蓟提取物对小鼠慢性酒精性肝损伤具有一定的保护作用,其机制可能与抗氧化、调控脂质代谢及调控Bax/Bcl-2/Csapase-3信号通路抑制肝脏细胞凋亡有关。Objectivee To study protective effect and mechanism of Cirsium tianmushanicum Shih extract(CSE)on chronic alcoholic liver injury in mice.Methods 36 healthy C57/BL6 mice were randomly divided into normal,model,BIF,CSE-1,CSE-m and CSE-h groups,6 mice each group.Physiological saline was given to normal group while 50%ethanol was given to the other groups.Ih later,mice in normal group and model group were given physiological saline,and mice in the other groups were given corresponding drugs.30 d later,the liver indexand the serum and liver biochemical indexes of mice were detected.Pathological changes of liver and small intestine,lipid accumulation in the liver and apoptosis of liver cells were respectively detected.The expression levels of proteins on the Bax/Bcl-2/Caspase-3 signal path were detected by Western Blotting.Results Compared with model group,the liver index,the activitiy of AST,ALT and the content of MDA and TG of Cirsium tianmushanicum Shih extract group mice were significantly decreased(P<0.01)while the activities of GSH were significantly increased(P<0.01).The morphology of liver and small intestine was significantly improved in the treatment group.It was showed that the apoptosis in liver tissue of each treatment group was significantly decreased.The expression of Bax,Cyt-C,Cleaved Caspase-3 proteins decreased(P<0.01),while the expression of Bcl-2 protein increased(P<0.01),and the expression of Caspase-3 protein had no difference.(P>0.01).Conclusion Cirsium tianmushanicum Shih extract can protect the chronic alcoholic liver injury,which may be due to anti-oxidation,regulation of lipid metabolism and inhibition of Csapase-3/Bax/Bcl-2 apoptosis pathway.

关 键 词：杭蓟 慢性酒精性肝损伤 氧化应激 脂质代谢 肝脏细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]