淫羊藿素通过调控IL-6/JAK2/STAT3途径抑制卵巢癌的发生与恶性生长  

Inhibition of Icaritin on Occurrence and Malignant Growth of Ovarian Cancer by Regulating the IL-6/JAK2/STAT3 Pathway

作　　者：何华 翁毅 余洁 何晓丹 伍桂 HE Hua;WENG Yi;YU Jie;HE Xiaodan;WU Gui(Suining Hospital of Traditional Chinese Medicine,Suining Sichuan 629000,China)

机构地区：[1]遂宁市中医院,四川遂宁629000

出　　处：《中医药导报》2024年第10期48-53,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：四川省科技厅重点研发项目(2021YFS0197)。

摘　　要：目的:探讨淫羊藿素通过调控IL-6/JAK2/STAT3信号通路对卵巢癌A2780和SKOV3细胞增殖、凋亡和侵袭及对小鼠卵巢癌移植瘤模型的影响。方法:将人卵巢癌A2780和SKOV3细胞置于细胞培养箱中培养,设置空白对照组和淫羊藿素低、中、高剂量组(5.00、10.00、20.00μmol/L),药物干预48 h。采用克隆形成实验检测细胞增殖,流式细胞术检测细胞凋亡,Transwell实验检测细胞侵袭。Western blotting检测Ki67、PCNA、cleaved Caspase-3、cleaved Caspase-9、E-cadherin、N-cadherin、VEGF、IL-6、p-JAK2和p-STAT3蛋白相对表达量。建立裸鼠卵巢癌移植瘤模型,检测移植瘤质量和体积,采用Western blotting检测Ki67、cleaved Caspase-3、E-cadherin、N-cadherin、VEGF、IL-6、p-JAK2和p-STAT3蛋白的表达量。结果:与空白对照组比较,淫羊藿素中剂量和高剂量治疗后,卵巢癌A2780和SKOV3细胞增殖、侵袭降低,凋亡增加,体内外Ki67、PCNA、cleaved Caspase-3、cleaved Caspase-9、N-cadherin、VEGF、IL-6、p-JAK2和p-STAT3蛋白的表达量下调,E-cadherin蛋白的表达量上调。结论:淫羊藿素可抑制卵巢癌A2780和SKOV3细胞的增殖和侵袭,促进细胞凋亡,其机制与淫羊藿素影响IL-6、JAK2和STAT3的表达有关。Objective:To investigate the effects of Icaritin on the proliferation,apoptosis and invasion of ovarian cancer A2780 and SKOV3 cells and the transplanted tumor model of ovarian cancer in mice by regulating the IL-6/JAK2/STAT3 signaling pathway.Methods:Human ovarian cancer A2780 and SKOV3 cells were cultured in a cell incubator.Blank control group,Icaritin low dose group(5μmol/L),Icaritin medium dose group(10μmol/L)and Icaritin high dose group(20μmol/L)were intervened with medication for 48 hours.Colony formation assay was used to detect cell proliferation,and flow cytometry was used to detect cell apoptosis.Transwell assay was used to detect cell invasion.The protein relative expressions of Ki67,PCNA,cleaved Caspase-3,cleaved Caspase-9,E-cadherin,N-cadherin,VEGF,IL-6,p-JAK2 and p-STAT3 were detected by Western blotting.The nude mouse ovarian cancer transplant tumor model has been established,and the weight and volume of the transplanted tumor were detected.The protein relative expressions of Ki67,cleaved Caspase-3,E-cadherin,N-cadherin,VEGF,IL-6,p-JAK2 and p-STAT3 were detected by Western blotting.Results:Compared with the blank control group,the proliferation and invasion of ovarian cancer A2780 and SKOV3 cells were decreased,and apoptosis was increased after treatment in Icaritin medium dose group and Icaritin high dose group.The protein relative expressions of Ki67,PCNA,cleaved Caspase-3,cleaved Caspase-9,N-cadherin,VEGF,IL-6,p-JAK2 and p-STAT3 were significantly down-regulated,while protein relative expressions of E-cadherin was significantly up-regulated in vivo and in vitro.Conclusion:Icaritin can inhibit the proliferation and invasion of ovarian cancer A2780 and SKOV3 cells,and promote cell apoptosis.The mechanism may be related to the effect on the expression of IL-6,JAK2 and STAT3.

关 键 词：卵巢癌 淫羊藿素 IL-6/JAK2/STAT3信号通路 裸鼠 

分 类 号：R285.5[医药卫生—中药学]