不同频率重复脉冲与短阵快速脉冲经颅磁刺激对小鼠记忆功能和神经元兴奋性影响的对比分析  

Comparative analysis of the impact of repetitive transcranial magnetic stimulation and burst transcranial magnetic stimulation at different frequencies on memory function and neuronal excitability of mice

作　　者：付蕊 朱海军 丁冲 徐桂芝[1,2] FU Rui;ZHU Haijun;DING Chong;XU Guizhi(Key Laboratory of Bioelectromagnetic and Neural Engineering of Hebei Province,Hebei University of Technology,Tianjin 300130,P.R.China;State Key Laboratory of Reliability and Intelligence of Electrical Equipment,Hebei University of Technology,Tianjin 300130,P.R.China;Key Laboratory of Digital Medical Engiineering of Hebei Province,Hebei University,Baoding,Hebei 071002,P.R.China)

机构地区：[1]河北工业大学河北省生物电磁与神经工程重点实验室,天津300130 [2]河北工业大学省部共建电工装备可靠性与智能化国家重点实验室,天津300130 [3]河北大学河北省数字医疗工程重点实验室,河北保定071002

出　　处：《生物医学工程学杂志》2024年第5期935-944,共10页Journal of Biomedical Engineering

基　　金：国家重点研发计划项目(2022YFC2402203);国家自然科学基金项目(52077057,52207251);河北省自然科学基金资助项目(E2021202222);天津市脑科学与神经工程重点实验室开放基金。

摘　　要：经颅磁刺激(TMS)作为一种无创的神经调控技术现已用于神经和精神类疾病的临床治疗,但不同频率与模式TMS的刺激效果及其神经调控机制尚未明确。本文从神经电生理角度探究不同频率重复TMS(rTMS)和短阵快速脉冲TMS(bTMS)对小鼠学习记忆和神经元兴奋性的影响。本文实验采用8周的昆明小鼠42只,随机分为伪刺激组和刺激组;刺激组包括:不同频率(1、5、10 Hz)的rTMS刺激组;不同频率(1、5、10 Hz)的bTMS刺激组。其中,刺激组接受连续14 d的刺激。刺激结束后,小鼠进行新物体识别与跳台实验以检测小鼠记忆能力;随后进行脑片膜片钳实验分析小鼠海马齿状回(DG)颗粒细胞兴奋性。结果表明,与伪刺激组相比,高频(5、10 Hz)的rTMS与bTMS能提高小鼠记忆能力和神经元兴奋性,而低频(1 Hz)的rTMS与bTMS则无显著效果;同频率下两种刺激模式对小鼠记忆功能与神经元兴奋性的影响无明显差别。本文研究结果提示,高频TMS可通过提高海马DG颗粒神经元兴奋性来提高小鼠记忆功能。本文为TMS改善认知功能的机制研究和临床应用提供实验和理论依据。Transcranial magnetic stimulation(TMS)as a non-invasive neuroregulatory technique has been applied in the clinical treatment of neurological and psychiatric diseases.However,the stimulation effects and neural regulatory mechanisms of TMS with different frequencies and modes are not yet clear.This article explores the effects of different frequency repetitive transcranial magnetic stimulation(rTMS)and burst transcranial magnetic stimulation(bTMS)on memory function and neuronal excitability in mice from the perspective of neuroelectrophysiology.In this experiment,42 Kunming mice aged 8 weeks were randomly divided into pseudo stimulation group and stimulation groups.The stimulation group included rTMS stimulation groups with different frequencies(1,5,10 Hz),and bTMS stimulation groups with different frequencies(1,5,10 Hz).Among them,the stimulation group received continuous stimulation for 14 days.After the stimulation,the mice underwent new object recognition and platform jumping experiment to test their memory ability.Subsequently,brain slice patch clamp experiment was conducted to analyze the excitability of granulosa cells in the dentate gyrus(DG)of mice.The results showed that compared with the pseudo stimulation group,high-frequency(5,10 Hz)rTMS and bTMS could improve the memory ability and neuronal excitability of mice,while low-frequency(1 Hz)rTMS and bTMS have no significant effect.For the two stimulation modes at the same frequency,their effects on memory function and neuronal excitability of mice have no significant difference.The results of this study suggest that high-frequency TMS can improve memory function in mice by increasing the excitability of hippocampal DG granule neurons.This article provides experimental and theoretical basis for the mechanism research and clinical application of TMS in improving cognitive function.

关 键 词：经颅磁刺激 新物体识别 跳台实验 颗粒细胞 脑片膜片钳实验 神经元兴奋性 

分 类 号：R318[医药卫生—生物医学工程]