基于大鼠脑片的经颅直流电刺激参数优化仿真研究  

Simulation study on parameter optimization of transcranial direct current stimulation based on rat brain slices

作　　者：何世纪 张广浩[1,2] 吴昌哲 霍小林 张李君[1,2] 张竞兮 张丞 HE Shiji;ZHANG Guanghao;WU Changzhe;HUO Xiaolin;ZHANG Lijun;ZHANG Jingxi;ZHANG Cheng(Beijing Key Laboratory of Bioelectromagnetism,Institute of Electrical Engineering,Chinese Academy of Sciences,Beijing 100190,P.R.China;School of Electronics,Electrical and Communication Engineering,University of Chinese Academy of Sciences,Beijing 100049,P.R.China)

机构地区：[1]中国科学院电工研究所生物电磁学北京市重点实验室,北京100190 [2]中国科学院大学电子电气与通信工程学院,北京100049

出　　处：《生物医学工程学杂志》2024年第5期945-950,共6页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(52077209,51977205);国家重点研发计划(2022YFC2402200)。

摘　　要：经颅直流电刺激(tDCS)是治疗精神疾病和神经退行性疾病的重要手段。本文分别通过大鼠脑片染色图像和磁共振成像(MRI)数据重建了两个离体脑片模型,并使用有限元计算得出tDCS皮层后大鼠脑区海马处的电流密度。随后,通过神经元模型计算这些电流密度下大鼠海马锥体神经元的响应,比较两种模型在不同刺激参数下的神经元反应。结果显示,基于脑片染色图像的模型中,刺激电压最小为17 V时可引起海马锥体神经元的兴奋,而基于MRI的模型需24 V。分析表明,基于脑片染色图像的模型在精细度和电场传播模拟方面更具优势,结果更接近真实测量值,可为tDCS参数选择提供指导,并为精准刺激提供科学依据。Transcranial direct current stimulation(tDCS)is an important method for treating mental illnesses and neurodegenerative diseases.This paper reconstructed two ex vivo brain slice models based on rat brain slice staining images and magnetic resonance imaging(MRI)data respectively,and the current densities of hippocampus after cortical tDCS were obtained through finite element calculation.Subsequently,a neuron model was used to calculate the response of rat hippocampal pyramidal neuron under these current densities,and the neuronal responses of the two models under different stimulation parameters were compared.The results show that a minimum stimulation voltage of 17 V can excite hippocampal pyramidal neuron in the model based on brain slice staining images,while 24 V is required in the MRI-based model.The results indicate that the model based on brain slice staining images has advantages in precision and electric field propagation simulation,and its results are closer to real measurements,which can provide guidance for the selection of tDCS parameters and scientific basis for precise stimulation.

关 键 词：经颅直流电刺激 大鼠脑片 刺激参数 

分 类 号：R318[医药卫生—生物医学工程] TP391.9[医药卫生—基础医学]