饲粮中添加酶制剂和抗菌肽对藏鸡肠道内源酶活性的影响  

Effects of dietary enzyme preparations and antimicrobial peptides on intestinal enzyme activities of Tibetan chickens

作　　者：王韩可 冯卫东 郑玉才[1] 李志雄 倪建华 饶开晴[1] Wang Hanke;Feng Weidong;Zheng Yucai;Li Zhixiong;Ni Jianghua;Rao Kaiqing(Sichuan Provincial Key Laboratory of Animal Medicine,Southwest Minzu University,Chengdu 610041,China;Sichuan Ganzi Tibetan Autonomous Prefecture Animal Husbandry Research Institute,Ganzi 626000,Sichuan Province,China;Xiangcheng County Zanggege Agricultural Development Co.,Ltd.,Ganzi 626700,Sichuan Province,China)

机构地区：[1]西南民族大学动物医学四川省高等学校重点实验室,四川成都610041 [2]四川省甘孜藏族自治州畜牧业科学研究所,四川甘孜626000 [3]乡城县藏咯咯农业开发有限公司,四川甘孜626700

出　　处：《天津农学院学报》2024年第5期46-51,共6页Journal of Tianjin Agricultural University

基　　金：西南民族大学研究生创新型科研项目(ZD2022809);四川省科技计划项目(2021YFYZ0031,2023NSFSC0236)。

摘　　要：试验旨在探究饲粮中添加非淀粉多糖(Non-starch polysaccharides,NSP)复合酶制剂、抗菌肽及酶肽复合剂对21~63日龄藏鸡小肠内源酶活性的影响。选取体重相近的14日龄藏鸡240只,随机分为4组,即基础饲粮组(对照组)、酶制剂组、抗菌肽组和酶肽复合组。预饲7天,42、63日龄采集藏鸡十二指肠、空肠、回肠肠道内容物,检测各组食糜中α-淀粉酶、脂肪酶、胰蛋白酶及糜蛋白酶活性。结果显示,与对照组相比,在42日龄时,酶肽复合添加显著提高了藏鸡小肠α-淀粉酶、糜蛋白酶活性及十二指肠和回肠脂肪酶活性(P<0.05);酶制剂显著提高了藏鸡十二指肠、回肠α-淀粉酶活性和空肠、回肠脂肪酶活性(P<0.05);在63日龄时,酶肽复合添加显著提高了藏鸡小肠糜蛋白酶活性,显著提高了十二指肠和回肠α-淀粉酶活性及十二指肠、空肠脂肪酶活性(P<0.05);酶制剂显著提高了藏鸡小肠脂肪酶活性及十二指肠、回肠α-淀粉酶活性(P<0.05);而抗菌肽对藏鸡小肠内源酶活性无显著影响(P>0.05)。结果表明,酶制剂单独添加及酶制剂和抗菌肽联合添加均能显著增强藏鸡小肠内源酶活性,且联合添加效果优于酶制剂单独添加。The purpose of this experiment was to investigate the effects of dietary supplementation of non-starch polysaccharide(NSP)complex enzyme preparation,antimicrobial peptide and enzyme peptide complex on intestinal enzyme activities of Tibetan chickens aged 21 to 63 days.A total of 24014-day-old Tibetan chickens with similar body weight were randomly divided into 4 groups:basal diet group(control group),enzyme preparation group,antimicrobial peptide group and enzyme peptide complex group.Pre-feed for 7 days,the contents of duodenum,jejunum and ileum of Tibetan chickens at 42 and 63 days of age were collected,and the activities ofα-amylase,lipase,trypsin and chymotrypsin in chymus of each group were detected.The results showed that,compared with the control group,the activities ofα-amylase and chylin in small intestine,duodenal and ileal lipase in Tibetan chickens at 42 days of age were significantly increased in the enzyme-peptide complex group(P<0.05);The enzyme preparation significantly enhanced theα-amylase activity in both the duodenum and ileum,the lipase activity of jejunum and ileum in Tibetan chickens(P<0.05);At 63 days of age,the activities of chymotrypsin,α-amylase and duodenal and jejunal lipase in duodenum and ileum of Tibetan chickens were significantly increased with the supplementation of enzyme peptide complex(P<0.05);The enzyme preparation significantly increased the activity of lipase in small intestine,α-amylase activity in duodenum and ileum of Tibetan chickens(P<0.05);However,antimicrobial peptides had no significant effect on intestinal enzyme activity of Tibetan chickens(P>0.05).The results showed that the enzyme preparation alone and the combination of enzyme preparation and antimicrobial peptide could significantly enhance the enzyme activity in small intestine of Tibetan chickens,and the combined addition effect was better than that of enzyme preparation alone.

关 键 词：酶制剂 抗菌肽 藏鸡 肠道内源酶 

分 类 号：S831.5[农业科学—畜牧学]