水稻对林丹的吸收累积与代谢组学研究  

Uptake and accumulation of lindane in rice and its metabolomics

作　　者：展梦琪 苏傲雪 侯倩 张皓宇 姜欣蕊 徐艳 ZHAN Mengqi;SU Aoxue;HOU Qian;ZHANG Haoyu;JIANG Xinrui;XU Yan*(School of Environmental Science and Engineering,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学环境科学与工程学院,山东青岛266071

出　　处：《浙江农业学报》2024年第9期2110-2121,共12页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(42177028);山东省高等学校青创人才引育计划(DC2000000961)。

摘　　要：以有机氯代污染物——林丹作为研究对象,研究其在我国典型农作物——水稻(Oryza sativa L.)体内的迁移转化和代谢情况。结果发现,在林丹胁迫条件下,水稻根和地上部的林丹含量与水培溶液中的林丹浓度具有正相关性,表明水稻具有吸收林丹的能力。在不同林丹水培浓度下,水稻根内的林丹累积浓度在第二天均达到峰值,第三天后急剧下降。水稻根系内的林丹可以径向运输至茎叶中,但转运因子仅为0.08~0.13。在亚细胞水平上,水稻根中林丹的含量从高到低依次为细胞器>细胞壁>细胞质,表明细胞器和细胞壁是水稻根部林丹累积的主要部位。代谢组学分析结果表明,林丹胁迫下,脂质类代谢物的变化最显著,有43种上调、42种下调。受林丹胁迫影响,水稻根中的异牡荆苷等代谢物的含量提高,而天冬酰胺等代谢物的含量降低,多条植物代谢通路被改变,其中,鞘脂代谢通路和苯丙氨酸代谢通路受到较大影响。The bioaccumulation,translocation and metabolomics of organochlorine pollutant lindane in the typical crop in China,rice(Oryza sativa L.)were investigated.The results revealed that under the stress of lindane contamination,the lindane concentration in rice roots,and above-ground parts(stems and leaves)had a positive correlation with the lindane concentration in the hydroponic solution,which indicated that rice had the ability to absorb lindane.Under different lindane concentrations in the hydroponic solution,the lindane accumulation in rice roots reached its peak on the second day and decreased sharply on the third day.In addition,lindane in rice roots could be transported radially to the above-ground parts,but the transport factor was 0.08-0.13.At subcellular level,the content of lindane in rice roots decreased as organelles>cytoderm>cytoplasm,which indicated that the organelle and cell wall were the main storage sites of lindane in rice roots.Under lindane stress,the changes of lipid metabolites were the most significant,with 43 up-regulated and 42 down-regulated.The content of isovitexin and other metabolites increased,while the content of asparagine and other metabolites decreased under lindane stres,and many metabolic pathways in plant were changed,among which,the sphingolipid metabolic pathway and phenylalanine metabolic pathway were greatly influenced.

关 键 词：林丹 水稻 植物代谢 吸收转运 农产品安全 

分 类 号：S511[农业科学—作物学] X503.231[环境科学与工程—环境工程]