云南番茄潜叶蛾捕食性天敌资源调查及其控害作用分子检测  

Investigation and Molecular Evaluation of the Natural Enemies of Tuta absoluta(Meyrick)(Lepidoptera:Gelechiidae)in Tomato Fields in Yunnan Province

作　　者：张桂芬[1] 朱华康 黄亮 王玉生[1,3] 李涛 黄聪[1] 冼晓青 薛延韬[1] 桂富荣 刘万学[1] 万方浩[1] 张毅波[1] ZHANG Guifen;ZHU Huangkang;HUANG Liang;WANG Yusheng;LI Tao;HUANG Cong;XIAN Xiaoqing;XUE Yantao;GUI Furong;LIU Wanxue;WAN Fanghao;ZHANG Yibo(State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Invasive Alien Species Management and Center for Management of Invasive Alien Species,Ministry of Agriculture and Rural Affairs/Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;College of Plant Protection,Hunan Agricultural University,Changsha 410125,China;Institute of Agricultural Sciences of the 14th Division of Xinjiang Production and Construction Corps,Hetian 848116,China)

机构地区：[1]中国农业科学院植物保护研究所/植物病虫害综合治理全国重点实验室/农业农村部外来入侵生物防控重点实验室/农业农村部外来入侵生物预防与控制研究中心,北京100193 [2]云南农业大学植物保护学院,昆明650201 [3]湖南农业大学植物保护学院,长沙410125 [4]新疆生产建设兵团第十四师农业科学研究所,和田848116

出　　处：《中国生物防治学报》2024年第4期739-749,共11页Chinese Journal of Biological Control

基　　金：国家重点研发计划(2021YFD1400200,2017YFC1200600);中国农业科学院科技创新工程(CAASCX-2019-2023-IAS,CAASZDRW202203);云南省生物多样性保护基金会资助项目(2023-2024)。

摘　　要：云南省是番茄潜叶蛾Tuta absoluta(Meyrick)较早入侵的区域,为明确本地潜在自然天敌资源及其对番茄潜叶蛾的控制作用,采用田间调查、样本采集以及与室内分子检测相结合的技术方法,于7—10月对云南省10个地级州市主要番茄种植区的番茄潜叶蛾天敌资源进行调查,对采集的天敌种类进行DNA条形码鉴定,对其控害作用进行种特异性分子检测。结果表明,在番茄田共计采集到捕食性天敌36种,隶属2纲5目16科。进一步的分子检测结果显示,对番茄潜叶蛾具有控害作用的捕食性自然天敌19种,隶属2纲3目12科;其中,昆虫纲8种、蜘蛛纲11种;对番茄潜叶蛾控害作用阳性检出率为5.7%~100.0%。分布范围最为广泛的天敌为半翅目盲蝽科的烟盲蝽Nesidiocoris tenuis Reuter,在8个州市有分布;其次为花蝽科的黑翅小花蝽Oirus agilis(Flor)和小花蝽Orius sp.以及瓢虫科的七星瓢虫Coccinella septempunctata L.,分别在5个、4个和5个州市有分布;再次为蜘蛛目狼蛛科的拟环纹豹蛛Pardosa pseudoannulata(Böesenberg et Strand),在3个州市有分布;而其余的14个物种仅在1个或2个州市有分布。番茄潜叶蛾天敌资源最为丰富的区域为昭通市,有8种天敌;其次为丽江市和玉溪市,分别有6种和5种天敌;再次昆明市和曲靖市,分别有4种和3种天敌;而大理州、文山市、红河州有2种天敌,楚雄市和普洱市只有1种天敌。综合评价分析结果显示,控害潜力最大的为烟盲蝽,其次为黑翅小花蝽;此外,七星瓢虫和拟环纹豹蛛也表现了较好的控害能力。番茄潜叶蛾在西南虽发生为害较为严重,但对该种害虫具有控制作用的天敌种类也比较多,为利用本地天敌防治番茄潜叶蛾提供了自然资源保障。Yunnan Province is one of the two earliest invaded areas of Tuta absoluta(Meyrick)(Lepidoptera:Gelechiidae)in China.The potential indigenous natural enemies of T.absoluta were investigated,collected,and identified during July to October in tomato planting in 10 prefectures of Yunnan Province.The species of collected natural enemies were identified using DNA barcoding technique and their control efficacy on T.absoluta in tomato fields were evaluated using T.absoluta species-specific PCR(SS-PCR)technique.Our results indicated that there were 36 predatory species detected in tomato fields,belonging to 16 families,5 orders,2 classes.The results of SS-PCR detection shown that 19 species,belonging to 12 families,3 orders,2 classes,fed on T.absoluta.Among the 19 predatory natural enemy species,8 species belong to Insecta and 11 species belong to Arachnida.The percentages of positive detection of T.absoluta were between 5.7%and 100.0%.Of the 19 predatory species,the most widely distributed was Nesidiocoris tenuis Reuter(Hemiptera:Miridae),which was detected in 8 prefectures,followed by Oirus agilis(Flor),Orius sp.(Hemiptera:Anthocoridae),and Coccinella septempunctata L.(Coleoptera:Coccinellidae),which were detected in 5,4,and 5 prefectures,respectively.Pardosa pseudoannulata(Böesenberg et Strand)(Araneae:Lycosidae)were detected in 3 prefectures and the other 14 predatory species were detected only in one or two prefectures.Of the 10 tomato planting prefectures,Zhaotong had the most abundant natural enemy species of T.absoluta,where 8 predatory species were identified,followed by Lijiang and Yuxi,where 6 and 5 predatory natural enemy species were identified,respectively.Four and three predatory natural enemy species were identified in Kunming and Qujing,respectively,two species identified in Dali,Wenshan,and Honghe,and only one species was identified in Chuxiong and Puer.Comprehensive evaluation,based on percentage of positive detection of T.absoluta,dominance of predatory natural enemy population,and distribution freque

关 键 词：番茄潜叶蛾 捕食性天敌 优势种 番茄田 蜘蛛 综合评价 

分 类 号：S476[农业科学—农业昆虫与害虫防治]