特基拉芽胞杆菌YB-1145的分离鉴定及其对小麦茎基腐病的生防效果评价  

Isolation and Identification of Bacillus tequilensis YB-1145 and Evaluation of Its Biocontrol Effects on Wheat Crown Rot

作　　者：刘青祥 徐文 董迁迁[1,2] 夏明聪 张洁[2] 孙润红[2] 武超[2] 吴坤[1] 杨丽荣[2] LIU Qingxiang;XU Wen;DONG Qianqian;XIA Mingcong;ZHANG Jie;SUN Runhong;WU Chao;WU Kun;YANG Lirong(College of Life Sciences,Henan Agricultural University,Zhengzhou 450046,China;Institute of Plant Protection Research,Henan Academy of Agricultural Sciences/Henan Agricultural Microbiology Innovation Center,Zhengzhou 450002,China)

机构地区：[1]河南农业大学生命科学学院,郑州450046 [2]河南省农业科学院植物保护研究所/河南省生物农药工程研究中心/河南省农用微生物创新中心,郑州450002

出　　处：《中国生物防治学报》2024年第4期884-893,共10页Chinese Journal of Biological Control

基　　金：河南省重大科技专项(221100110100);中原科技创新领军人才(234200510010);河南省农业科学院科技创新团队专项(2023TD15);河南省科技攻关计划项目(222102110072);河南省科技攻关项目(222102110094)。

摘　　要：为了寻找防治小麦茎基腐病生防菌株,本研究采用平板对峙法从小麦根际土壤中筛选到一株出对小麦茎基腐病具有优良防效的菌株YB-1145。该菌株对假禾谷镰孢等6种植物病原菌菌丝生长具有显著抑制作用,据其形态特征、生理生化指标及16S rDNA和gyrA鉴定结果表明,该菌株为特基拉芽胞杆菌Bacillus tequilensis。生防特性研究表明,YB-1145具有分泌β-1,3-葡聚糖酶、蛋白酶、IAA和溶解无机磷的能力。室内盆栽防病试验与对照相比,1×10^(8) cfu/mL YB-1145菌悬液处理对小麦茎基腐病的防治效果达到了76.1%,小麦根长提高了17.1%,株高提高了22.6%,鲜重提高了71.4%。YB-1145处理后小麦幼苗叶片PPO、CAT、PAL、POD和LOX等活性显著提高。以上研究表明,YB-1145菌株具有显著的抑菌促生等生防潜力。In order to identify a biocontrol strain for wheat crown rot,strain YB-1145 was isolated from the rhizosphere soil of wheat using the plate confrontation method.This strain exhibited significant inhibition of mycelium growth in six plant pathogens,including Fusarium pseudograminearum.Based on its morphological characteristics,physiological and biochemical indicators,16S rDNA and gyrA identification results,the strain was identified as Bacillus tequilensis.Biocontrol studies have demonstrated that YB-1145 has the capacity to secreteβ-1,3-glucanase,protease,IAA and solubilize inorganic phosphorus.Compared to the control,inoculation with YB-1145 suspension of 1×10^(8) cfu/mL resulted in 76.1%reduction of wheat crown rot,17.1%increase of root length,22.6%increase of plant height,and 71.4%increase of fresh weight.Following treatment with YB-1145,activities of PPO,CAT,PAL,POD and LOX in wheat seedling leaves were significantly enhanced.These findings indicate that the YB-1145 strain possesses substantial biocontrol potential through pathogen inhibition and growth promotion.

关 键 词：小麦茎基腐病 假禾谷镰孢 特基拉芽胞杆菌 生防作用 

分 类 号：S476[农业科学—农业昆虫与害虫防治]