SMARTdb:An Integrated Database for Exploring Single-cell Multi-omics Data of Reproductive Medicine  

作　　者：Zekai Liu Zhen Yuan Yunlei Guo Ruilin Wang Yusheng Guan Zhanglian Wang Yunan Chen Tianlu Wang Meining Jiang Shuhui Bian 

机构地区：[1]State Key Laboratory of Reproductive Medicine and Offspring Health,School of Public Health,Nanjing Medical University,Nanjing 211166,China [2]Collaborative Innovation Center for Cancer Personalized Medicine,School of Public Health,Nanjing Medical University,Nanjing 211166,China

出　　处：《Genomics, Proteomics & Bioinformatics》2024年第3期113-123,共11页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by the Young Elite Scientists Sponsorship Program by China Association for Science and Technology(Grant No.2020QNRC001);the research start-up funding from Nanjing Medical University,China(Grant No.KY116RC20200007).

摘　　要：Single-cell multi-omics sequencing has greatly accelerated reproductive research in recent years,and the data are continually growing.However,utilizing these data resources is challenging for wet-lab researchers.A comprehensive platform for exploring single-cell multi-omics data related to reproduction is urgently needed.Here,we introduce the single-cell multi-omics atlas of reproduction(SMARTdb),an integrative and user-friendly platform for exploring molecular dynamics of reproductive development,aging,and disease,which covers multi-omics,multi-species,and multi-stage data.We curated and analyzed single-cell transcriptomic and epigenomic data of over 2.0 million cells from 6 species across the entire lifespan.A series of powerful functionalities are provided,such as“Query gene expression”,“DIY expression plot”,“DNA methylation plot”,and“Epigenome browser”.With SMARTdb,we found that the male germ cell-specific expression pattern of RPL39L and RPL10L is conserved between human and other model animals.Moreover,DNA hypomethylation and open chromatin may collectively regulate the specific expression pattern of RPL39L in both male and female germ cells.In summary,SMARTdb is a powerful platform for convenient data mining and gaining novel insights into reproductive development,aging,and disease.SMARTdb is publicly available at https://smart-db.cn.

关 键 词：Database Single-cell multi-omics EMBRYO Germ cell GONAD 

分 类 号：Q811.4[生物学—生物工程]