复方扶芳藤合剂调节免疫抑制小鼠外周血CD19^(+)B细胞CD69和PD-1的表达  

Compound fufangteng mixture regulates CD69 and PD-1 expressions of peripheral CD19~+B cells in immunosuppressed mice

作　　者：彭丽珊[1] 陈果 李建明 杨洋[1] 刘显[1] 肖健[1] PENG Li-shan;CHEN Guo;LI Jian-ming;YANG Yang;LIU Xian;XIAO Jian(Guangxi Key Laboratory of Integrative Translational Medicine for High Incidence Infectious Diseases,Guangxi University of Traditional Chinese Medicine,Nanning 530001,China;Department of Spleen and Stomach Diseases,Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430060,China)

机构地区：[1]广西中医药大学,广西高发传染病中西医结合转化医学重点实验室,南宁530001 [2]湖北省中医院脾胃病科,武汉430060

出　　处：《现代免疫学》2024年第5期407-412,共6页Current Immunology

基　　金：国家自然科学基金(81860780);广西自然科学基金面上项目(2020GXNSFAA297162);广西壮族自治区中医药管理局基金项目(GXZYA20220087)。

摘　　要：为评估复方扶芳藤合剂(compound fufangteng mixture,CFM)对环磷酰胺诱导的免疫抑制BALB/c小鼠B细胞的免疫增强作用,将BALB/c小鼠随机分为空白对照(normal control,NC)组、模型(model control,MC)组、CFM低剂量(low-dose CFM,LD)组、CFM中剂量(medium-dose CFM,MD)组、CFM高剂量组(high-dose CFM,HD)组和阳性对照(positive control,PC)组。采用FACS检测小鼠外周血和脾脏CD19^(+)B细胞表面活化分子(CD69、CD38)和耗竭分子(PD-1、CD95)的表达。结果显示,与NC组比较,MC组外周血CD19^(+)B细胞百分比显著下降(P<0.05),LD组、MD组、HD组外周血CD19^(+)B细胞百分比有改善的趋势,并随着CFM剂量的递增而升高;LD组、MD组外周血CD19^(+)B细胞CD69和PD-1表达水平显著高于NC组(P<0.05);与外周血CD19^(+)B细胞比较,脾脏中CD19^(+)B细胞CD69、CD38、PD-1和CD95的表达水平在各组间差异无统计学意义(P>0.05)。该研究提示,CFM对小鼠外周血CD19^(+)B细胞可能具有改善作用,且对其CD69和PD-1有调节作用。This study aims to evaluate the immune-enhancing effect of compound fufangteng mixture(CFM)on B cells in cyclophosphamide-induced immunosuppression in BALB/c mice.BALB/c mice were randomly divided into normal control(NC)group,model control(MC)group,low-dose CFM(LD)group,medium-dose CFM(MD)group,high-dose CFM(HD)group,and positive control(PD)group.The expressions of CD19~+B cell surface activating molecules(CD69,CD38)and exhaustion molecules(PD-1,CD95)in mice peripheral blood and spleen were detected by FACS.The results showed that compared to that of the NC group,the percentage of CD19~+B cells in the peripheral blood of MC group decreased significantly(P<0.05),while the percentages of CD19~+B cells in the peripheral blood of LD group,MD group,and HD group increased in a CFM dose-dependent manner.The expressions of CD69 and PD-1 of CD19~+B cells in the peripheral blood of LD group and MD group were significantly higher than those of the NC group(P<0.05).There was no significant difference in the expression levels of CD69,CD38,PD-1,and CD95 of splenetic CD19~+B cells among the six groups(P>0.05).Altogether,the results suggest that the CFM may improve the percentage of peripheral blood CD19~+B cells and regulate CD69 and PD-1 in immunosuppression mice.

关 键 词：复方扶芳藤合剂 免疫抑制 CD19^(+)B细胞 CD69 程序性死亡受体1 

分 类 号：R285.5[医药卫生—中药学]