雷公藤甲素调控lncRNA BE503655影响骨肉瘤细胞SaOS-2增殖、侵袭和凋亡  

The effect of triptolide on the proliferation,invasion and apoptosis of osteosarcoma cell SaOS-2 through lncRNA BE503655

作　　者：白洋 付丽丽[1] 姬晓蕊 黄纬[2] 李向辉[1] 褚桂克[1] 徐娜[1] 金杰[3] BAI Yang;FU Lili;JI Xiaorui;HUANG Wei;LI Xianghui;CHU Guike;XU Na;JIN Jie(Department of Traditional Chinese Medicine,Nanyang Central Hospital,Nanyang 473000,China;Nanyang Medical College,Nanyang 473000,China;The First Affiliated Hospital Of Henan University of Chinese Medicine,Henan 450000,China)

机构地区：[1]南阳市中心医院中医科,河南南阳473000 [2]南阳医学高等专科学校,河南南阳473000 [3]河南中医药大学第一附属医院,河南郑州450000

出　　处：《沈阳药科大学学报》2024年第10期1339-1347,1368,共10页Journal of Shenyang Pharmaceutical University

基　　金：河南省中医药科学研究专项课题(2019JDZX2080)。

摘　　要：目的研究雷公藤甲素对骨肉瘤细胞SaOS-2增殖、侵袭和凋亡的影响和机制.方法骨肉瘤细胞SaOS-2分成Control组、Triptolide组(40、80、160 nmol·L^(-1)雷公藤甲素)、阳性对照组(15 mg·L^(-1)5-氟尿嘧啶)、Triptolide+si-NC组(转染siRNA control,160 nmol·L^(-1)雷公藤甲素)、Triptolide+si-BE503655组(转染BE503655 siRNA,160 nmol·L^(-1)雷公藤甲素),以CCK-8实验分析细胞变化,用流式细胞术分析细胞凋亡变化,以Transwell小室分析细胞迁移和侵袭变化,West-ern blot分析神经性钙黏附素(neural cadherin,N-cadherin)、上皮性钙黏附素(epithelical cadherin,E-cadherin)、Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)、Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)、基质金属蛋白酶-2(matrix metalloprotease 2,MMP-2)蛋白表达变化,qRT-PCR方法分析BE503655表达.结果与Control组比较,Triptolide组(40、80、160 nmol·L^(-1)雷公藤甲素)、阳性对照组骨肉瘤细胞存活率降低,细胞凋亡率升高,侵袭数目和迁移数目减少,细胞中Bax、E-cadherin蛋白表达水平增加,Bcl-2、MMP-2、N-cadherin蛋白表达水平降低,BE503655表达水平升高.与Triptolide+si-NC组比较,Triptolide+si-BE503655组骨肉瘤细胞存活率升高,细胞凋亡率降低,迁移和侵袭数目均增加,MMP-2、N-cadherin、Bcl-2蛋白表达水平均升高,E-cadherin、Bax蛋白表达水平均降低,BE503655水平降低.结论雷公藤甲素通过上调BE503655发挥抑制骨肉瘤细胞SaOS-2增殖、迁移、侵袭、EMT和促凋亡作用.Objective To study the effect of triptolide on the proliferation,invasion and apoptosis of osteosarcoma cell SaOS-2.Methods SaOS-2 osteosarcoma cells were divided into Control group,Triptolide group(40,80,160 nmol·L^(-1) triptolide),positive control group(15 mg·L^(-1)5-fluorouracil),Triptolide+si-NC group(transfected siRNA control,160 nmol·L^(-1) triptolide),Triptolide+si-BE503655 group(transfected with BE503655 siRNA,160 nmol·L^(-1) triptolide),CCK-8 experiment was used to analyze cell changes,flow cytometry was used to analyze cell apoptosis,and Transwell chamber was used to analyze cell migration and invasion changes,Western blot was used to analyze N-cadherin,E-cadherin,Bax,Bcl-2,MMP-2 protein expression changes,qRT-PCR method was used to analyze the expression of BE503655.Results Compared with the control group,the survival rate of osteosarcoma cells in the Triptolide group(40,80,160 nmol·L^(-1) triptolide)and positive control group were decreased,the apoptosis rate was increased,and the number of invasion and migration were decreased.Bax,E-cadherin protein expression level were increased,Bcl-2,MMP-2,N-cadherin protein expression level was decreased,and BE503655 expression level was increased.Compared with the Triptolide+si-NC group,the osteosarcoma cell survival rate in the Triptolide+si-BE503655 group was increased,the apoptosis rate was decreased,the number of migration and invasion were increased,and the protein expression levels of MMP-2,N-cadherin and Bcl-2 were increased,and the expression levels of E-cadherin and Bax protein were decreased,the BE503655 level was decreased.Conclusion Triptolide exerts the effects of inhibiting proliferation,migration,invasion,EMT and promoting apoptosis of osteosarcoma cell SaOS-2 by up-regulating BE503655.

关 键 词：骨肉瘤 雷公藤甲素 侵袭 凋亡 BE503655 

分 类 号：R96[医药卫生—药理学]