基于UPLC-MS/MS技术对榼藤不同药用部位成分分析及其止痒镇痛活性评价  

Component analysis of different medicinal parts of Entada phaseoloides based upon UPLC-MS/MS technology and evaluations of antipruritic and analgesic activities

作　　者：王梦玲 黄菊 韩学斌 谭艳颖 熊慧[1] 梅之南 WANG Mengling;HUANG Ju;HAN Xuebin;TAN Yanying;XIONG Hui;MEI Zhinan(College of Pharmacy,Central South Minzu University,Hubei Wuhan 430074,China;College of Plant Science&Technology,Huazhong Agricultural University,Hubei Wuhan 430070,China)

机构地区：[1]中南民族大学药学院,湖北武汉430074 [2]华中农业大学植物科学技术学院,湖北武汉430070

出　　处：《中国医院药学杂志》2024年第19期2230-2235,2249,共7页Chinese Journal of Hospital Pharmacy

基　　金：国家重点研发计划项目(编号:2022YFC3502200)。

摘　　要：目的:比较榼藤Entada phaseoloides(Linn.)Merr.种仁、种皮和藤茎等不同部位的化学成分和药效差异,探讨成分与药效之间的关系。方法:应用超高效液相色谱串联质谱(UPLC-MS/MS)技术结合主成分分析、正交偏最小二乘法判别分析等多元分析方法,鉴定并比较榼藤不同部位的成分组成和差异。建立组胺或氯喹诱导的小鼠急性瘙痒模型和弗氏完全佐剂诱导的大鼠炎性痛模型,评价榼藤不同部位的止痒和镇痛活性。并通过皮尔逊相关系数分析差异化合物与止痒、镇痛药效间的关联性。结果:从榼藤中共鉴定出85个化合物,其中51个化合物为榼藤不同部位间差异的主要变量。药效结果显示,榼藤种仁止痒作用强于种皮和藤茎,种仁和藤茎的镇痛效果较种皮好。通过相关性分析得出,与止痒作用相关的有epigallocatechin-3-O-gallate(51)、cynaroside(64)等4个化合物;与镇痛作用相关的有eriodictyol(5)、3',4',7-trihydroxyflavone(11)、3-O-[β-D-xylopyranosyl-(1→2)-α-L-arabinopyranosyl-(1→6)]-2-acetylamino-2-deoxy-β-D-glucopyranosylentagenic acid(20)和cynaroside(64)等11个化合物。结论:该研究对榼藤不同部位的化学成分进行了较全面的分析以及止痒和镇痛的活性评价。其中,黄酮类和三萜类成分与药效相关性较高。OBJECTIVE To compare the variations of chemical constituents and pharmacodynamics of different parts from Entada phaseoloides(Linn.)Merr.,including seed kernels,seed coats and stems,and investigate the correlations between chemical constituents and pharmacodynamics.METHODS Chemical components and different parts of E.phaseoloides were examined through UPLC-MS/MS,principal analysis,partial least square discriminant analysis and other stoichiometrics.Histamine or chloroquine-evoked acute pruritus model in mice and complete Freund's adjuvant-induced inflammatory pain model in rats were employed for evaluating the antipruritic and analgesic activities of different parts of E.phaseoloides.Furthermore,the correlation between differential compounds and anti-inflammatory,antipruritic and analgesic activities was verified by Pearson's correlation analysis.RESULTS A total of 85 compounds were identified,including flavonoids,phenolic acids,triterpenes and entadamides,etc.Among them,51 compounds were the major variables among different parts of E.phaseoloides.Additionally,seed kernels of E.phaseoloides displayed more dramatic antipruritic effect.And both seed kernels and stems exhibited marked analgesic activity.Moreover,according to the results of correlation analysis,4 compounds,including epigallocatechin-3-O-gallate(51)and cynaroside(64),contributed significantly to antipruritic effect while 11 compounds containing eriodictyol(5),3',4',7-trihydroxyflavone(11),3-O-[β-D-xylopyranosyl-(1→2)-α-L-arabinopyranosyl-(1→6)]-2-acetylamino-2-deoxy-β-D-glucopyranosylentagenic acid(20)and cynaroside(64)were associated with analgesic activities.CONCLUSION This study has comprehensively compared the chemical constituents in different parts of E.phaseoloides and evaluated the antipruritic and analgesic activities.And the flavonoids and triterpenoids in E.phaseoloides are associated with pharmacological properties.

关 键 词：榼藤 药用部位 化学成分 镇痛作用 止痒作用 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学] R961