UHPLC-Q-Exactive Orbitrap HRMS技术分析肾衰乙方的化学成分与大鼠口服后的组织分布  

Chemical profiling and tissue distribution of Shenshuai Yifang after oral dosing in rats by UHPLC-QExactive-Orbitrap HRMS

作　　者：沈沁 李荣胜 段连香 曹玉 杨翊清 樊叶楠 翁涛涛 刘伟[2] 胡静[1] SHEN Qin;LI Rongsheng;DUAN Lianxiang;CAO Yu;YANG Yiqing;FAN Yenan;WENG Taotao;LIU Wei;HU Jing(Department of Nephropathy,Seventh People’s Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200137,China;Department of Pharmacy,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,The NATCM Third Grade Laboratory of Traditional Chinese Medicine Preparations,Institute of Liver Diseases,Key Laboratory of Liver and Kidney Diseases(Ministry of Education),Shanghai Key Laboratory of Traditional Chinese Clinical Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属第七人民医院肾病科,上海200137 [2]上海中医药大学附属曙光医院药学部,国家中医药管理局中药制剂三级实验室,肝肾疾病病证教育部重点实验室,上海市中医临床重点实验室,上海201203

出　　处：《中国医院药学杂志》2024年第19期2250-2255,共6页Chinese Journal of Hospital Pharmacy

基　　金：浦东新区卫生健康委员会卫生计生科研项目(编号:PW2022D-12);浦东新区中医专病品牌倍增计划-慢性肾病专病(编号:PDZY-2021-0302);何立群名中医工作室建设(编号:PDZY-2022-0703)。

摘　　要：目的:运用UHPLC-Q-Exactive-Orbitrap HRMS技术分析鉴定肾衰乙方的化学成分,以及大鼠灌胃后主要入血、组织原型成分分布。方法:采用Acquity UPLC BEH C18色谱柱(100 mm×2.1 mm,1.7μm),以0.1%甲酸水溶液(A)-甲醇(B)为流动相体系,梯度洗脱分离。质谱采用正负离子一级全扫描/数据依赖二级扫描模式(Full MS/dd-MS2)采集数据,经Xcalibur 4.2软件获取色谱保留时间以及准分子离子碎片信息,通过对照品比对、文献参考以及自建数据库等成分检测信息,实现对肾衰乙方化学成分及入血、组织原型成分的鉴定。结果:共鉴定出166种化学成分,包括黄酮类56种、皂苷类25种、蒽醌类24种、有机(酚)酸类23种、苯酞类14种、生物碱和环肽类各7种、木脂素类和香豆素类各3种以及其他类4种。大鼠口服肾衰乙方后分别从血清、心、肝、脾、肺、肾、脑、睾丸中检测到54、53、61、47、71、48、53、42种原型成分。结论:该研究利用高分辨质谱技术完成了肾衰乙方化学成分及主要入血、组织移行成分的快速鉴定,可为肾衰乙方效应物质的解析及作用机制研究提供参考。OBJECTIVE To employ UHPLC-Q-Exactive-Orbitrap HRMS to identify the chemical components of Shenshuai Yifang and explore the distribution of major components in blood and tissues after oral dosing in rats.METHODS An Acquity UPLC BEH C18 chromatographic column(100 mm×2.1 mm,1.7μm)was utilized as solid phase while mobile phase composed of 0.1%formic acid(A)and methanol(B)with gradient elution.Mass spectra were collected with a Full scan of positive and negative ions/data-dependent on secondary scan function(Full MS/dd-MS2)for data collection.Chromatographic retention time and quasi-molecular ion fragment information were acquired by Xcalibur 4.2 software while identification of components was compared with reference standards,literature reference and self-established database.RESULTS A total of 166 chemical components were identified from Shenshuai Yifang,including 56 kinds of flavonoids,25 kinds of saponins,24 kinds of anthraquinones,23 kinds of organic and phenolic acids,14 kinds of phthalides,7 kinds of cyclic peptides and alkaloids,3 kinds of lignans and coumarins and 4 kinds of others.After oral dosing of Shenshuai Yifang to rats,54,53,61,47,71,48,53,42 prototypic components were detected in sera,heart,liver,spleen,lung,kidney,brain and testicles respectively.CONCLUSION In this study,the chemical components and major blood/tissue distribution components of Shenshuai Yifang,have been rapidly identified by high-resolution mass spectrometry.It may provide references for analyzing effective substances and elucidating the action mechanism of Shenshuai Yifang.

关 键 词：UHPLC-Q-Exactive Orbitrap HRMS 肾衰乙方 化学成分 入血成分 组织分布 

分 类 号：R284.1[医药卫生—中药学] R965[医药卫生—中医学]