新型不完全溶血表型金黄色葡萄球菌的微生物学特征  

Characterization of Staphylococcus aureus strains with novel incomplete hemolytic phenotype

作　　者：唐伟 冷贵云[1] 高菊 王亚武 姚杰 周强[1] 徐元宏[2] Tang Wei;Leng Guiyun;Gao Ju;Wang Yawu;Yao Jie;Zhou Qiang;Xu Yuanhong(Dept of Clinical Laboratory,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601;Dept of Clinical Laboratory,The First Affiliated Hospital of Anhui Medical University,Hefei 230022)

机构地区：[1]安徽医科大学第二附属医院检验科,合肥230601 [2]安徽医科大学第一附属医院检验科,合肥230022

出　　处：《安徽医科大学学报》2024年第9期1535-1542,共8页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金项目(编号:82102460)。

摘　　要：目的探讨一种新型不完全溶血表型金黄色葡萄球菌(SIHP)的微生物学特征。方法采用三点接种法检测溶血表型,共纳入11株新型SIHP和33株随机匹配的完全溶血表型金黄色葡萄球菌(SCHP)。采用微量肉汤稀释法检测新型SIHP和SCHP的耐药特征,冻干兔血浆检测凝固酶活性,玻片法检测触酶活性,实时荧光定量PCR检测溶血素基因mRNA水平,微孔板法检测红细胞毒性,微孔板成膜结晶紫染色法检测生物膜形成能力,微量培养法监测生长曲线。结果与SCHP相比,新型SIHP的hla、hlb、hlc和hld四种溶血素基因表达谱不同;新型SIHP对青霉素、苯唑西林、庆大霉素、喹诺酮类、克林霉素和复方磺胺甲口恶唑的耐药率更高;新型SIHP不仅具有更强的溶血毒性、血浆凝固酶活性和生物膜形成能力,而且对数期生长更快。结论新型SIHP的微生物学特征有别于SCHP,耐药性和致病性更强,应引起临床重视。Objective To explore the microbiological characteristics of Staphylococcus aureus(S.aureus)with novel incomplete hemolytic phenotype(SIHP).Methods Hemolytic phenotypes were detected and categorized by using the three-point inoculation method.A total of 11 novel SIHP and 33 randomly matched S.aureus with complete hemolytic phenotype(SCHP)were included.Antibiotic susceptibility test was performed using broth microdilution method.Coagulase test was performed with freeze-dried rabbit plasma.Catalase activity was detected by slide catalase test.Expression of hemolysin genes was detected by qRT-PCR.Toxicity to human red blood cells was assessed by microplate method.Microplate biofilm formation was measured using crystal violet staining method.Growth kinetic determination was performed through microcultivation assay.Results Compared with SCHP,the expression profiles of the four hemolysin genes(hla,hlb,hlc,and hld)in the new SIHP were different.The new SIHP had higher resistance rates to penicillin,oxacillin,gentamicin,quinolones,clindamycin,and trimethoprim-sulfamethoxazole.Furthermore,the new SIHP had stronger hemolytic toxicity,plasma coagulase activity,and biofilm formation ability.Additionally,the new SIHP grown faster in the logarithmic phase.Conclusion Taken together,the microbiological characteristics of the new SIHP are different from those of SCHP,including stronger antibiotic resistance and pathogenicity,which should be paid more attention by clinicians.

关 键 词：金黄色葡萄球菌 溶血素 溶血表型 微生物学特征 耐药性 致病性 

分 类 号：R378.1[医药卫生—病原生物学]