基于不同透气性的包装容器单因素设计探索广陈皮陈化标志物  

Mining of Biomarkers for the Ageing of Citri Reticulatae'Chachi'Pericarpium by Single Factor Design for Packaging Containers with Different Breathability

作　　者：陈明权 商雪莹 李华[2] 覃仁安 何风雷 CHEN Ming-quan;SHANG Xue-ying;LI Hua;QIN Renan;HE Feng-ei(Guangzhou Baiyunshan Chenliji Pharmaceutical Co.,Ltd.,Guangzhou 510288,China;Guangdong Institute for Drug Control,Guangzhou 510663,China)

机构地区：[1]广州白云山陈李济药厂有限公司,广东广州510288 [2]广东省药品检验所,广东广州510663

出　　处：《中国现代中药》2024年第10期1672-1677,共6页Modern Chinese Medicine

基　　金：国家药品监督管理局药品快速检验技术重点实验室开放性课题(KF2021005);广州市科技计划项目(201710010122)。

摘　　要：目的:研究反映广陈皮陈化特征的标志物。方法:将同一来源的广陈皮在不同透气性的包装容器内放置3年,以模拟出不同陈化等级的样品;采用高效液相色谱-二极管阵列检测器法(HPLC-DAD)对不同陈化等级广陈皮样品进行全波长(200~400 nm)扫描,分析样品之间存在差异的特征性色谱峰;利用硅胶柱色谱法、半制备型HPLC进行分离纯化,通过核磁共振波谱、质谱法对化合物进行结构鉴定。结果:广陈皮中间苯三酚-O-葡萄糖苷与陈化等级呈负相关,即陈化等级越高,该成分含量越低;5-羟甲基-2-呋喃甲酸、香草酸与陈化等级呈正相关,即陈化等级越高,2个成分含量越高。结论:间苯三酚-O-葡萄糖苷、5-羟甲基-2-呋喃甲酸、香草酸3个成分均可作为反映广陈皮陈化特征的标志物,为阐述广陈皮陈化过程原理及量化广陈皮陈化度提供参考。Objective:To study the biomarkers reflecting the ageing characteristics of Citri Reticulatae'Chachi'Pericarpium.Methods:First,the Citri Reticulatae'Chachi'Pericarpium samples with the same source were placed in the packaging containers with different breathability for three years to simulate the samples with different ageing levels.Then,high performance liquid chromatography-diode array detector(HPLC-DAD)was employed to perform full wavelength(200-400 nm)scanning on the samples,and the characteristic chromatographic peaks with differences between samples were analyzed.Finally,silica gel column chromatography and semi-preparative high-performance liquid chromatography were used for separation and purification,and the structures of compounds were identified by nuclear magnetic resonance spectroscopy and mass spectrometry.Results:The content of phloroglucinol-O-glucoside showed a negative relationship with the ageing level,which indicated that the sample with higher ageing level had lower content of this component.The content of 5-hydroxymethyl-2-furancarboxylic acid and vanillic acid was positively correlated with the ageing level,which indicated that the sample with higher ageing level had higher content of the two components.Conclusion:Phloroglucinol-O-glucoside,5-hydroxymethyl-2-furancarboxylic acid,and vanillic acid can serve as biomarkers reflecting the ageing characteristics of Citri Reticulatae'Chachi'Pericarpium.The results lay a foundation for elucidating the principle of the ageing process and quantifying the aging degree of this herbal medicine.

关 键 词：广陈皮 陈化 间苯三酚-O-葡萄糖苷 5-羟甲基-2-呋喃甲酸 香草酸 

分 类 号：R284[医药卫生—中药学]