输精后注射hCG、GnRH对奶牛受胎率的影响  

Effects of injection of hCG and GnRH after insemination on the first service conception in cows

作　　者：王晋 王春涛 王月 刘鑫 李万超 张海博 WANG Jin;WANG Chuntao;WANG Yue;LIU Xin;LI Wanchao;ZHANG Haibo(Beijing SUNLON Livestock Development Co.,Ltd.,Beijing 100171,China)

机构地区：[1]北京首农畜牧发展有限公司,北京100171

出　　处：《畜牧与兽医》2024年第11期33-36,共4页Animal Husbandry & Veterinary Medicine

基　　金：现代农业产业技术体系项目(CARS-36);北京三元种业科技有限公司自立科研课题(SYZYZ20210013)。

摘　　要：为提高人工授精后荷斯坦奶牛头胎受胎率,研究肌注绒毛膜促性腺激素(hCG)和促性腺激素释放激素(GnRH)的最佳时间和浓度,持续跟踪8头奶牛在输精后30 d内血清中的孕酮浓度变化,每2 d检测1次,分析峰值出现时间;在每个峰值出现前1 d,分别对奶牛肌注1 000 IU hCG或100μg GnRH(n=30),并设置相应空白对照(n=30),分析各组受胎率;选择受胎率高的处理组奶牛,在授精后13 d分别注射1 000、2 000和3 000 IU的hCG(n=30),分析对应受胎率。结果显示:孕酮在授精后2、6和14 d出现3个峰值,分别是1 479、1 472和1 431 pmol/L。在授精后1、5、13 d,肌注GnRH组的受胎率分别是34.5%(10/29)、46.7%(14/30)、40.0%(12/30),对照组为39.3%(11/28),组间无显著性差异(P=0.836);肌注hCG组受胎率分别是21.4%(6/28)、37.9%(11/29)、58.6%(17/29),对照组为30%(9/30),其中授精后1 d与授精后13 d存在显著性差异(P<0.05)。授精后13 d,肌注hCG的1 000、2 000和3 000 IU梯度试验受胎率分别为50.0%(15/30)、56.7%(17/30)、80.0%(24/30),其中肌注1 000 IU与3 000 IU组存在显著性差异(P<0.05)。综上,授精后13 d肌注3 000 IU hCG能显著提高头胎奶牛受胎率。In order to increase the first-service conception rate in Holstein cows after artificial insemination,the optimal time and concentration of intramuscular injection of hCG and GnRH were studied here.Progesterone concentration in the serum of 8 cows was checked every two days within 30 days after insemination so as to analyze the peak value.1000 IU hCG or 100μg GnRH were injected one day before the peak,respectively(n=30),and the corresponding control group(n=30)was established to analyze the conception rates.Finally,on the 13th day after insemination,the cows were injected with different doses of hCG(1000,2000,and 3000 IU)to analyze the corresponding conception rates(n=30).The results were as follows:Progesterone exhibited three peaks at 2,6,and 14 days after insemination,which were 1479,1472,and 1431 pmol/L,respectively.Following this,one group of cows were injected with 1000 IU hCG on the first,fifth,and thirteenth day after insemination,while another group of cows were injected with 100μg GnRH at the same time.The conception rates of the GnRH group were 34.5%(10/29)on day 1,46.7%(14/30)on day 5,and 40.0%(12/30)on day 13;which were of no significant difference with those of the control group(39.3%(11/28),P=0.836).The conception rates of the hCG group were 21.4%(6/28)on day 1,37.9%(11/29)on day 5,and 58.6%(17/29)on day 13;that of the control group was 30%(9/30),and there was a significant difference(P<0.05)between day 1 and day 13 after pregnancy.The pregnancy rates of hCG injection on the 13-day after insemination were 50.0%(15/30)for 1000 IU,56.7%(17/30)for 2000 IU,and 80.0%(24/30)for 3000 IU.There was a significant difference between 1000 and 3000 IU(P<0.05).In summary,intramuscular injection of 3000 IU hCG on day 13 after artificial insemination significantly raised the first-serve conception rate in cows.

关 键 词：奶牛受胎率 孕酮 绒毛膜促性腺激素 促性腺激素释放激素 

分 类 号：S823[农业科学—畜牧学]