若羌红枣多糖铜螯合物的制备及生物活性检测  

Preparation and biological activity detection of copper chelate from Ruoqiang red jujube polysaccharides

作　　者：希尔艾力·阿不力克木 德力拜尔·木拉提 张佳怡 孟研 阿得力江·吾斯曼 刘丹丹 郭庆勇[1] 赛福丁·阿不拉[1] Xieraili Abulikemu;Delibaier Mulati;ZHANG Jiayi;MENG Yan;Adelijiang Wusiman;LIU Dandan;GUO Qingyong;Saifuding Abula(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China;Animal Epidemic Disease Prevention and Control Center of Xinjiang Uygur Autonomous Region,Urumqi 830011,China)

机构地区：[1]新疆农业大学动物医学学院,新疆乌鲁木齐830052 [2]新疆维吾尔自治区动物疫病预防控制中心,新疆乌鲁木齐830011

出　　处：《畜牧与兽医》2024年第11期63-71,共9页Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发计划项目(2021YFD1600702-3);新疆维吾尔自治区自然科学计划(少数民族特培)项目(390000017)。

摘　　要：采用柠檬酸钠-硫酸铜方法制备若羌红枣多糖铜螯合物(RJP-Cu),并对其含量、表征、安全性、补铜效果及免疫增强活性进行综合检测分析,其中安全性试验选择36只SPF级昆明(KM)小鼠,随机分为空白对照组(生理盐水)和灌胃2300、1150、575、288、144 mg/kg RJP-Cu试验组。结果显示:RJP-Cu与若羌红枣多糖(RJP)相比,多糖含量、蛋白含量未发生明显变化,糖醛酸含量下降,铜含量从2 mg/kg提升至583 mg/kg,RJP-Cu电镜结构发生明显变化,RJP-Cu同时具有多糖和铜螯合吸收峰;安全性评价结果显示,RJP-Cu的安全使用浓度为575 mg/kg,该浓度下小鼠血常规各项指标与空白对照组无显著性差异(P>0.05),肝脏、肾脏和肠道无病理变化。RJP-Cu补铜效果呈浓度依赖性,随着RJP-Cu浓度提高,血清、肝脏和肌肉中铜浓度提升;RJP-Cu低剂量(144 mg/kg)组相对空白对照组能够显著促进小鼠免疫球蛋白(Ig)A、IgG、白细胞介素(IL)-2、IL-6、肿瘤坏死因子α(TNF-α)的分泌(P<0.05),表现出良好的免疫增强活性。结论:RJP-Cu螯合成功,144 mg/kg低剂量的RJP-Cu具有良好的免疫增强作用。his study was to prepare copper chelates of polysaccharides from Ruoqiang red jujube(RJP-Cu)and detect their biological activity.RJP-Cu was prepared using the sodium citrate copper sulfate method,and comprehensive analysis was conducted on various contents,characterization,safety,copper supplementation effect,and immune enhancement activity of the copper chelates.In the safety test,36 SPF KM mice were selected and randomly divided into blank control group(normal saline)and 2300,1150,575,288,144 mg/kg RJP-Cu experimental groups.The results showed that,compared with RJP,the polysaccharide content and protein content in RJP-Cu did not change,the uronic acid content in it decreased,the Cu content increased from 2 mg/kg to 583 mg/kg,the electron microscope structure of RJP-Cu changed significantly,and RJP-Cu had both polysaccharide and Cu chelating absorption peaks.The safety test results showed that the safe concentration of RJP-Cu was 575 mg/kg.At this concentration,there was no significant difference in blood routine indexes compared with the blank control group of mice(P>0.05),and no pathological changes in the liver,kidney and intestine of the rodents.The effect of RJP-Cu supplementation was concentration-dependent.With the increase in RJP-Cu concentration,the Cu concentration in the serum,liver and muscle of the animals increased.The low-dose(144 mg/kg)RJP-Cu group significantly promoted the secretion of IgA,IgG,IL-2,IL-6 and TNF-αin the tested mice,compared with the blank control group(P<0.05),which exhibited good immune-enhancing activities.These results indicated that RJP-Cu chelated successfully,and the low dose(144 mg/kg)of RJP-Cu had a good immune enhancement effect.

关 键 词：若羌红枣 多糖 铜螯合物 免疫增强 

分 类 号：S826.9[农业科学—畜牧学]