乳杆菌胞外多糖纳米佐剂对鸡血清4型禽腺病毒亚单位疫苗的免疫增强作用  

Immune enhancement of Lactobacillus extracellular polysaccharide nanoadjuvant on fowl adenovirus serotype 4 subunit vaccine in chicken

作　　者：张硕 姬贺龙 丁晨晨 宋素泉[1] 闫丽萍 ZHANG Shuo;JI Helong;DING Chenchen;SONG Suquan;YAN Liping(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学动物医学院,江苏南京210095

出　　处：《畜牧与兽医》2024年第11期72-79,共8页Animal Husbandry & Veterinary Medicine

基　　金：泰州市“接榜挂帅”重点科技项目(JBGS202103)。

摘　　要：为了开发安全有效的新型兽用疫苗佐剂,将乳杆菌胞外多糖与季铵化壳聚糖自组装制备出两种纳米颗粒佐剂(NPs 7-4和NPs 8-2),研究其对蛋白抗原的免疫增强效果。纳米颗粒佐剂与血清4型禽腺病毒(FAdV-4)五邻体蛋白(Penton base)亚单位疫苗颈部皮下免疫接种7日龄SPF鸡21 d后肌肉注射JS株FAdV-4,测定组织器官病毒载量和泄殖腔排毒量,白油佐剂作为对照。结果显示:两种纳米颗粒佐剂对Penton base亚单位疫苗均具有缓释作用;与白油佐剂组相比,NPs 8-2佐剂组极显著提高了免疫鸡的血清特异性抗体水平(P<0.001),而NPs 7-4佐剂组抗体水平无显著差异(P>0.05);攻毒保护试验结果表明,与白油佐剂相比,NPs 8-2佐剂组提高了疫苗的攻毒保护率,组织脏器损伤明显减轻,组织病毒载量和泄殖腔排毒量显著减少(P<0.05),而NPs 7-4佐剂组免疫增强作用不显著。综上,NPs 8-2佐剂能有效增强血清4型禽腺病毒Penton base亚单位疫苗的免疫效果,减少抗原蛋白用量,产生较好的免疫保护效果,其免疫增强效果明显优于传统白油佐剂。In order to develop safe and effective novel veterinary vaccine adjuvants,two nanoparticle adjuvants(NPs 7-4 and NPs 8-2)were prepared in this study by self-assembly of Lactobacillus extracellular polysaccharide and quaternized chitosan,and the immune-enhancing effects of the NPs against protein antigens were investigated.Seven-day-old SPF chickens were used and inoculated subcutaneously with NPs adjuvants and Penton base subunit vaccine of serotype 4 avian adenovirus(FAdV-4)in the neck for 21 days,followed by intramuscular injection of FAdV-4 JS strain;and tissue organ viral loads and cloacal effluxes in the chickens were determined.At the same time,oil adjuvant was used as a control.The results showed that both NPs 7-4 and NPs 8-2 had a sustained release effect on FAdV-4 Penton base subunit vaccine.Compared with the white oil adjuvant group,the immune group with NPs 8-2 adjuvant significantly improved the serum specific antibody level of the immunized chickens(P<0.001),but the NPs 7-4 adjuvant immune group showed no significant difference(P>0.05).The results of the virus challenge experiment showed that,compared with the white oil adjuvant,the NPs 8-2 adjuvant improved the protection rate,mitigated the degree of tissue damage,and significantly reduced the virus load in the tissues and the cloacal swab excretion(P<0.05)in the birds,whereas the immune enhancing effect of the NPs 7-4 adjuvant was not significant.In summary,NPs 8-2 adjuvant could effectively enhance the immune effect of the FAdV-4 Penton base subunit vaccine,reduce the dosage of antigen protein,and produce a better immune-protection effect.The immune enhancing effect of NPs 8-2 adjuvant was significantly better than that of traditional oil adjuvants.

关 键 词：乳杆菌胞外多糖 纳米佐剂 血清4型禽腺病毒 免疫效果 

分 类 号：S852.6[农业科学—基础兽医学]