玉米肽对酒精损伤PC12细胞的保护作用及机制  

Protective Effect and Mechanism of Corn Peptide on Alcohol-Induced Injury in PC12 Cells

作　　者：林巍 韩思琪 刘晓兰 许英一 LIN Wei;HAN Siqi;LIU Xiaolan;XU Yingyi(Heilongjiang Key Laboratory of Corn Deep Processing Theory and Technology,College of Food and Bioengineering,Qiqihar University,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学食品与生物工程学院,黑龙江省玉米深加工理论与技术重点实验室,黑龙江齐齐哈尔161006

出　　处：《食品科学》2024年第21期194-202,共9页Food Science

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(145109317);中央引导地方科技发展专项(ZY23QY06)。

摘　　要：在细胞水平上探讨玉米肽对酒精损伤PC12细胞的保护作用及可能机制。首先建立酒精损伤细胞模型,对玉米肽的作用剂量进行筛选;采用酶联免疫吸附试剂盒法测定玉米肽预处理后PC12细胞中氧化应激指标(谷胱甘肽(glutathione,GSH)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA))、炎症相关因子(肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、核因子-κB(nuclear factor-κB,NF-κB))、神经营养因子(神经生长因子(nerve growth factor,NGF)、脑源性神经营养因子(brain-derived neurotrophic factor,BDNF))、突触可塑性相关蛋白(突触后致密物质-95(postsynaptic density-95,PSD-95)、生长相关蛋白-43(growth-associated protein-43,GAP-43)、突触素(synaptophysin,SYN))等指标。通过细胞存活率和形态变化,选择400 mmol/L为酒精损伤的最适浓度,确定玉米肽剂量低、中、高质量浓度为25、100、400μg/mL;实验结果表明3个剂量组的玉米肽对酒精损伤的PC12细胞存活率都具有显著的提高作用;氧化应激指标发现玉米肽可提高酒精损伤PC12细胞中GSH和SOD含量、抑制MDA的生成;炎症相关指标测定结果表明玉米肽可降低酒精损伤PC12细胞中炎症因子TNF-α和IL-6的水平,抑制乙醇导致的NF-κB含量升高;对神经营养因子含量的测定表明玉米肽还可提高酒精损伤细胞中NGF和BDNF的含量;此外玉米肽还可以增加突触可塑性相关蛋白PSD-95和GAP-43的含量,但对SYN无改善作用。总体来看,玉米肽对酒精损伤PC12细胞具有保护作用,其保护作用机制为多种途径共同作用的结果。The protective effect and possible mechanism of corn peptide on alcohol-induced injury in PC12 cells were explored.A cell model of alcohol-induced injury was established,and different concentrations of corn peptide were screened.Oxidative stress indicators such as glutathione(GSH),superoxide dismutase(SOD),malondialdehyde(MDA),inflammatory cytokines such as tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and nuclear factor-κB(NF-κB),neurotrophic factors such as nerve growth factor(NGF),brain-derived neurotrophic factor(BDNF)and synaptic plasticity-associated proteins including postsynaptic density-95(PSD-95),growth-associated protein-43(GAP-43)and synaptophysin(SYN)in PC12 cells pretreated with corn peptide were determined using an enzyme-linked immunosorbent assay kit.The optimal alcohol concentration for inducing cell damage was determined to be 400 mmol/L based on cell viability and morphological changes,and three concentrations of corn peptide,25(low),100(medium),and 400(high)μg/mL,were selected for this study.The experimental results showed that corn peptide at all three concentrations significantly increased the survival of alcoholinjured PC12 cells,increased the levels of GSH and SOD,inhibited MDA production,reduced the levels of TNF-αand IL-6,inhibited the increase in NF-κB levels induced by alcohol,increased the levels of NGF and BDNF,and elevated PSD-95 and GAP-43 levels,but had no regulatory effect on SYN levels.In conclusion,corn peptide has a protective effect on alcoholinduced injury in PC12 cells,and the mechanism involves multiple pathways.

关 键 词：玉米肽 酒精损伤 PC12细胞 保护作用 

分 类 号：TS201.4[轻工技术与工程—食品科学]