葛根素调控Mzb1对内质网应激介导心肌细胞线粒体功能障碍的影响  

Effects of Mzb1 regulation by puerarin on endoplasmic reticulum stress-mediated mitochondrial dysfunction in cardiomyocytes

作　　者：张棋 张悦 顾晶 孙佳希 王乐韬 张璐 ZHANG Qi;ZHANG Yue;GU Jing;SUN Jiaxi;WANG Letao;ZHANG Lu(Scientific Experiment Center of Shenyang Medical College,Liaoning,Shenyang 110034,China;The First Clinical Medical College of Shenyang Medical College,Liaoning,Shenyang 110034,China)

机构地区：[1]沈阳医学院科学实验中心,辽宁沈阳110034 [2]沈阳医学院第一临床医学院,辽宁沈阳110034

出　　处：《中国医药科学》2024年第19期4-7,共4页China Medicine And Pharmacy

基　　金：国家自然科学基金(82100316);辽宁省科学技术计划项目(2022-BS-342)。

摘　　要：目的观察葛根素对衣霉素诱导的心肌细胞线粒体功能障碍的影响。方法培养AC16人心肌细胞,采用衣霉素诱导内质网应激,葛根素作用于心肌细胞,通过Lipofectamine®3000脂质体转染siRNA Mzb1(si-Mzb1)。MTT法用于细胞活力的检测;ATP试剂盒用于检测各组内ATP含量变化;JC-1染色用于检测线粒体膜电位的变化;蛋白质印迹法用于检测边缘区B和B1细胞特异性蛋白(Mzb1),内质网应激相关蛋白葡萄糖调节蛋白78(GRP78)、CCAAT/增强子结合蛋白的同源蛋白(CHOP)的表达水平。结果与对照组比较,衣霉素组细胞活力下降(P<0.01),内质网应激相关蛋白GRP78、CHOP表达升高(P<0.05、P<0.01),Mzb1表达显著降低(P<0.05),ATP含量及线粒体膜电位下降(均P<0.05);与衣霉素组比较,葛根素组细胞活力有所升高(P<0.05),GRP78、CHOP蛋白表达下降(均P<0.05),Mzb1表达升高(P<0.05),ATP含量及线粒体膜电位部分恢复(均P<0.05);转染si-Mzb1后,葛根素的作用被阻断(均P<0.05)。结论葛根素调控Mzb1改善内质网应激导致的心肌细胞线粒体功能障碍。Objective To observe the effect of puerarin on the mitochondrial function in cardiomyocytes induced by tunicamycin.Methods AC16 human cardiomyocytes were cultured.The endoplasmic reticulum stress was induced by tunicamycin.Puerarin was applied to cardiomyocytes.siRNA Mzb1(si-Mzb1)was transfected by Lipofectamine®3000 liposomes.MTT assay was used to detect cell viability.ATP kit was used to detect changes in ATP content within each group.JC-1 staining was used to detect changes in mitochondrial membrane potential.Protein blotting was used to detect the expression level of marginal zone B and B1 cell-specific protein(Mzb1)and endoplasmic reticulum stress-related proteins,including glucose-regulated protein 78(GRP78)and CCAAT/enhancer-binding protein homologous protein(CHOP).Results Compared with the control group,tunicamycin induced a decrease in cell viability(P<0.01),an increase in the expression of endoplasmic reticulum stress-related proteins GRP78 and CHOP(P<0.05,P<0.01),a significant decrease in the expression of Mzb1(P<0.05),and a decrease in the ATP content and mitochondrial membrane potential(all P<0.05).Compared with the tunicamycin group,the cell viability was elevated(P<0.05),the expression of GRP78 and CHOP proteins was decreased(all P<0.05),the expression of Mzb1 was elevated(P<0.05),and the ATP content and mitochondrial membrane potential were partially restored(all P<0.05)in the puerarin group.However,the effect of puerarin was blocked after the transfection of si-Mzb1(all P<0.05).Conclusion Puerarin regulates Mzb1 to improve the endoplasmic reticulum stress-induced mitochondrial dysfunction in cardiomyocytes.

关 键 词：葛根素 AC16心肌细胞 衣霉素 内质网应激 线粒体功能 Mzb1 

分 类 号：R96[医药卫生—药理学]