盐酸小檗碱对人结肠癌细胞SW480增殖、迁移及侵袭的影响  

Effect of berberine hydrochloride on proliferation,migration and invasion of human colon cancer cell SW480

作　　者：尤玲玲 于思柔 蒋小哲 梁燕凤 郭荣 彭彤 华欣怡 尹茉莉 刘磊[1] YOU Lingling;YU Sirou;JIANG Xiaozhe;LIANG Yanfeng;GUO Rong;PENG Tong;HUA Xinyi;YIN Moli;LIU Lei(Jilin Medical University,Jilin,Jilin 132013,China)

机构地区：[1]吉林医药学院,吉林吉林132013

出　　处：《中国医药科学》2024年第19期17-20,共4页China Medicine And Pharmacy

基　　金：吉林省科技发展计划项目(20230203099SF);吉林省中医药科技项目(2022087);吉林医药学院博士启动基金(JYBS2021001LK);吉林省大学生创新创业训练计划项目(S202213706030)。

摘　　要：目的探究盐酸小檗碱对人结肠癌细胞SW480增殖、迁移和侵袭过程的影响及可能的机制。方法将人结肠癌细胞SW480细胞分成实验组、对照组、空白组,用CCK8法测定不同浓度的盐酸小檗碱对SW480细胞增殖的作用,并计算出盐酸小檗碱不同浓度作用下SW480细胞的存活率;通过平板克隆形成、细胞划痕、Transwell等实验,明确盐酸小檗碱对SW480的克隆形成、迁移及侵袭的作用。结果盐酸小檗碱能明显地抑制SW480细胞的增殖,并表现出明显的时间和浓度依赖关系;盐酸小檗碱对SW480细胞的克隆形成、迁移及侵袭过程有显著的抑制作用,差异有统计学意义(P<0.05)。结论盐酸小檗碱对细胞SW480的克隆形成、增殖、侵袭和迁移过程均有明显的抑制作用。Objective To explore the effect of berberine hydrochloride on the proliferation,migration and invasion of human colon cancer cell SW480 and its possible mechanism.Methods The human colon cancer cell SW480 was divided into experimental group,control group and blank group.The effects of berberine hydrochloride with different concentrations on the proliferation of SW480 cells were determined by CCK8 method,and the survival rate of SW480 cells were calculated.The effects of berberine hydrochloride on the clone formation,migration and invasion of SW480 were confirmed by experiments such as plate clone formation,cell scratch and Transwell.Results Berberine hydrochloride could obviously inhibit the proliferation of SW480 cells in a time and concentration dependent manner.Berberine hydrochloride significantly inhibited the formation,migration and invasion of SW480 cells,and the difference was statistically significant(P<0.05).Conclusion Berberine hydrochloride has an obvious inhibitory effect on the cloning,proliferation,invasion and migration of cell SW480.

关 键 词：盐酸小檗碱 人结肠癌 CCK8 细胞划痕 TRANSWELL 

分 类 号：R735.35[医药卫生—肿瘤]