基于胃微观动力结构研究整肠散干预兔功能性胃排空障碍的药效作用机制  

Study on the pharmacological mechanism of Zhengchang San in intervening functional gastric emptying disorders in rabbits based on the micro dynamic structure of the stomach

作　　者：危椠罡[1] 朱千虑 黄沐晗 张清花 陈水凤 许碧香 任新生[4] WEI Qiangang;ZHU Qianlv;HUANG Muhan;ZHANG Qinghua;CHEN Shuifeng;XU Bixiang;REN Xinsheng(Department of Nursing,the People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fujian,Fuzhou 350004,China;School of Nursing,Fujian University of Traditional Chinese Medicine,Fujian,Fuzhou 350108,China;Department of Respiratory,the People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fujian,Fuzhou 350004,China;Department of Cardiovascular Medicine,the People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fujian,Fuzhou 350004,China)

机构地区：[1]福建中医药大学附属人民医院护理部,福建福州350004 [2]福建中医药大学护理学院,福建福州350108 [3]福建中医药大学附属人民医院呼吸科,福建福州350004 [4]福建中医药大学附属人民医院心内科,福建福州350004

出　　处：《中国医药科学》2024年第19期21-26,共6页China Medicine And Pharmacy

基　　金：福建省自然科学基金(2020J011051);国家中医临床研究基地专项科研课题(JDZX201928)。

摘　　要：目的探讨整肠散穴位贴敷干预功能性胃排空障碍(FDGE)的可能药效作用机制。方法将30只雄性家兔按随机数表法分为正常组、模型组和穴位贴敷组,每组各10只。正常组行假手术,模型组和穴位贴敷组家兔行胃大部分切除术进行造模。正常组与模型组给予无药穴位贴敷,穴位贴敷组行整肠散穴位贴敷,各组均干预14 d。检测术日术前及术后第15日家兔血钾(K+)、胃动素(MTL)水平;术后第16日通过测定胃内色素残留量反映残胃排空功能;术后第16日运用电镜行残胃平滑肌细胞及Cajal间质细胞(ICC)的超微结构测定。结果与正常组比较,模型组MTL、K^(+)的含量显著降低(P<0.001),与模型组比较,穴位贴敷组MTL、K^(+)的含量显著提高(P<0.001);与正常组比较,模型组胃内色素残留量升高(P<0.001);与模型组比较,穴位贴敷组胃内色素残留量显著降低(P<0.001)。与正常组比较,模型组细胞核排列紊乱,形态不规则,ICC细胞结构模糊,核固缩,线粒体少,出现空泡。而与模型组比较,穴位贴敷组细胞破损较轻,排列较整齐,ICC细胞结构较清晰,突起增多,未见自噬小体,提示整肠散穴位贴敷能够改善兔胃黏膜细胞损伤,改善胃大部分切除术后家兔平滑肌细胞中ICC的超微结构。结论整肠散穴位贴敷能够有效预防家兔胃大部分切除术后FDGE的发生,其作用机制可能与稳定K^(+)水平,促进MTL分泌,提高胃排空功能,改善残胃平滑肌细胞与ICC的超微结构有关。Objective To explore the possible pharmacological mechanism of acupoint application of Zhengchang San in improving intervention for functional delayed gastric emptying(FDGE).Methods A total of 30 male rabbits were randomly divided into a normal group,a model group,and an acupoint application group,with 10 rabbits in each group.The normal group underwent sham surgery,while the rabbits in model group and acupoint application group underwent subtotal gastrectomy for modeling.The normal group and the model group were given non-medicine acupoint application,while the acupoint application group was treated with acupoint application of Zhengchang San.Each group was intervened for 14 days.The levels of potassium(K^(+))and motilin(MTL)in blood of rabbits were detected before and on the 15th day after surgery.On the 16th day after surgery,the amount of residual pigment in the stomach was measured to reflect residual gastric emptying function;on the 16th day after surgery,the ultrastructure of residual gastric smooth muscle cells and interstitial cells of cajal(ICC)was determined using electron microscopy.Results Compared with the normal group,the levels of MTL and K^(+)in the model group were significantly reduced(P<0.001).Compared with the normal.group,the amount of residual pigments in the stomach of model group increased(P<0.001).Compared with the model group,the amount of residual pigments in the stomach of acupoint application group was significantly reduced(P<0.001).Compared with then ormal group,the amount of residual pigments in the stomach of model group increased(P<0.001).Compared with the model group,the amount of residual pigments in the stomach of acupoint application group was significantly reduced(P<0.001).Compared with the normal group,the model group had disordered nuclear arrangement,irregular morphology,blurred ICC cell structure,nuclear condensation,fewer mitochondria,and the appearance of vacuoles.Compared with the model group,the acupoint application group hadless cell da mage,more orderly a rrangemen

关 键 词：功能性胃排空障碍 整肠散 穴位贴敷 CAJAL间质细胞 超微结构 

分 类 号：R248.9[医药卫生—中医临床基础]