牛羊布鲁氏菌天然半抗原琼脂扩散试验的建立与应用  

Establishment and application of natural hapten agar diffusion test for brucella

作　　者：刘丽娅[1] 剡文亮[2] 曹瑞 叶锋[1] 马晓菁[1] 谷文喜[1] 赵江山[4] 张子荣[5] 宋洁 李岩[2] 易新萍[1] LIU Liya;YAN Wenliang;CAO Rui;YE Feng;MA Xiaojing;GU Wenxi;ZHAO Jiangshan;ZHANG Zirong;SONG Jie;LI Yan;YI Xinping(Xinjiang Key Laboratory of Animal Infectious Diseases,Institute of Veterinary Medicine,Xinjang Academy of Animal Sciences,Urumqi 830011,China;The General Station of Animal Husbandry and Veterinary of Xinjiang Production and Construction Corps,Urumqi 830021,China;Qingdao Real Bio-Technology Co.,Ltd,Qingdao Shandong 266000,China;Centre for Disease Control and Prevention of Xinjiang Uygur Autonomous Region,Urumqi 830000,China;Animal Husbandry and Veterinary Work Station of the Sixth Division,Xinjiang Production and Construction Corps,Wujiaqu Xinjiang 831304,China;Xinjiang Tianrun Dairy limited Co.,Ltd,Urumqi 830000,China)

机构地区：[1]新疆动物疫病研究重点实验室/新疆畜牧科学院兽医研究所,乌鲁木齐830011 [2]新疆生产建设兵团畜牧兽医工作总站,乌鲁木齐830021 [3]青岛瑞尔唯特生物技术有限公司,山东青岛266000 [4]新疆维吾尔自治区疾病预防控制中心,乌鲁木齐830000 [5]新疆生产建设兵团第六师畜牧兽医工作站,新疆五家渠831304 [6]新疆天润乳业股份有限公司,乌鲁木齐830000

出　　处：《新疆农业科学》2024年第8期2063-2070,共8页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区重点研发专项(2022B03013-1);新疆生产建设兵团重点领域科技攻关计划项目(2020AB015)。

摘　　要：【目的】建立并优化布鲁氏菌天然半抗原琼脂扩散试验(Native hapten agar gel immuno-diffusion test,NH-AGID),检测临床样品。【方法】建立NH-AGID方法,评价方法特异性、敏感性及重复性;检测免疫地区2287份、非免疫地区252份牛、羊血清。【结果】建立了NH-AGID方法,检测稳定,重复性好,具有较高的特异性;对自然感染阳性动物检测敏感性低于80%,对排菌动物的检测敏感性高于90%,检出布病阳性抗体的阈值高于RBT。免疫地区血清NH抗体平均检出率17.8%,LPS抗体平均检出率52.3%;非免疫地区血清NH抗体平均检出率40.9%,LPS抗体平均检出率54.8%。【结论】应用NH-AGID方法可鉴别区分免疫地区牛、羊布病感染动物和免疫动物。检出NH抗体动物处于布鲁氏菌活动期或排菌期为布病感染动物,应及时剔除出群。【Objective】To establish and optimize the natural hapten agar gel immuno-diffusion test(NH-AGID)for Brucella abortus in clinical samples.【Methods】The NH-AGID method was established to evaluate the specificity,sensitivity and reproducibility of the method;2,287 bovine and sheep sera from immunized areas and 252 from non-immunized areas were tested.【Results】The NH-AGID method with stable and reproducible assay and high specificity was successfully established;The sensitivity of the assay for naturally infected positive animals was lower than 80%;The sensitivity of the assay for releasing animals was higher than 90%;The threshold for detecting bruclosis-positive antibodies was higher than RBT.The average detection rate of serum NH antibodies in immunized areas was 17.8%,and the average detection rate of LPS antibodies was 52.3%;the average detection rate of serum NH antibodies in non-immunized areas was 40.9%,and the average detection rate of LPS antibodies was 54.8%.【Conclusion】The NH-AGID method can be applied to identify and distinguish bovine and sheep brucellosis-infected animals from immunized animals in immunized areas.Animals detected with NH antibodies in the active or excretory phase of Brucella abortus are brucellosis-infected animals and should be removed from the herd in time.

关 键 词：布鲁氏菌病 半抗原-琼脂扩散试验 诊断 

分 类 号：S855[农业科学—临床兽医学]