电针促进脂肪干细胞在体成骨分化改善骨质疏松的机制  

Mechanism of electroacupuncture regulating Wnt/β-catenin to promote osteogenic differentiation of adipose-derived stem cells in vivo and improve osteoporosis

作　　者：郭佳[1] 舒晴 张思钰 赵娟 田峻[1] GUO Jia;SHU Qing;ZHANG Siyu;ZHAO Juan;TIAN Jun(Department of Rehabilitation,Zhongnan Hospital of Wuhan University,Wuhan 430061,China)

机构地区：[1]武汉大学中南医院康复医学科,湖北武汉430061

出　　处：《中国骨质疏松杂志》2024年第10期1412-1419,共8页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金面上项目(82174494)。

摘　　要：目的 探讨电针(electroacupuncture, EA)联合自体脂肪干细胞(adipose derived stem cells, ADSCs)移植对去卵巢大鼠骨质疏松的作用及对Wnt/β-catenin信号通路的影响。方法 将雌性SD大鼠随机分为假手术组、模型组、EA组、ADSCs组和EA+ADSCs组,每组10只。采用大鼠卵巢切除建立模型,在造模手术后2个月开始干预,EA组予双侧“脾俞”“肾俞”电针治疗,频率2 Hz,连续波,强度1 mA。每次10 min,每周3次,连续8周。ADSCs组将绿色荧光蛋白标记的ADSCs经尾静脉移植体内。每次1×10~6个,每周1次,连续8周。EA+ADSCs组予电针治疗,联合自体ADSCs移植,干预连续8周。检测大鼠股骨骨微结构及骨组织病理变化、血清骨代谢指标,股骨Wnt3a、β-catenin和Runx2基因及蛋白表达,及Runx2/β-catenin蛋白的共表达。结果 与假手术组比较,模型组大鼠骨密度显著降低(P<0.01),骨小梁间距变大;与模型组比较,EA组大鼠骨密度显著增高(P<0.01),骨小梁数量和厚度增加,Wnt3a、β-catenin、Runx2 mRNA和蛋白表达水平明显升高(P<0.01),Runx2/β-catenin蛋白表达显著升高(P<0.01);ADSCs组Runx2的mRNA和蛋白表达水平显著升高(P<0.01)。EA+ADSCs组骨小梁结构均优于ADSCs组和EA组,并且Runx2/β-catenin蛋白表达显著升高(P<0.01)。结论 ADSCs移植联合电针可以有效改善卵巢摘除大鼠模型骨质疏松状况,这种作用可能和激活Wnt/β-catenin信号通路有关。Objective To observethe effects of electroacupuncture combined with autologous adipose stem cells transplantation on osteoporosis and on the Wnt/β-catenin signaling pathway in ovariectomized rats.Methods Female SD rats were divided into sham operation group,model group,EA group,transplantation group and EA+ADSCs transplantation group,with 10 rats in each group.Two months after modeling,EA was applied to bilateral“Pishu”and“Shenshu”in the EA group,with oscillatory wave,2 Hz in frequency and 1 mA in intensity.Each session lasted 10 minutes,3 times a week for 8 weeks.The ADSCs group transplanted green fluorescent protein-labeled ADSCs in vivo via the tail vein,1×106 each time,1 time per week for 8 weeks.The EA+ADSCs group was given electroacupuncture treatment,combined with ADSCs transplantation,for 8 weeks.Detection of morphological and structural changes in rat femur tissue,serum bone metabolism indexes,as well as detection of femur Wnt3a,β-catenin and Runx2 gene expression and proteins,and co-expression of Runx2/β-catenin proteins.Results Compared with the sham-operated group,rats in the model group had significantly lower BMD(P<0.01)and larger spacing of bone trabeculae;compared with the model group,rats in the EA group had significantly higher BMD(P<0.01),increased number and thickness of bone trabeculae,and significantly higher expression levels of Wnt3a,β-catenin,and Runx2 mRNAs and proteins(P<0.01),and significantly higher Runx2/β-catenin fluorescence intensity was significantly higher(P<0.01);the expression level of Runx2 was significantly higher in the ADSCs group(P<0.01).The bone trabecular structure of the EA+ADSCs group was superior to that of both the ADSCs group and the EA group,and the Runx2/β-catenin fluorescence intensity was significantly higher(P<0.01).Conclusion ADSCs transplantation combined with electroacupuncture can effectively improve osteoporosis in an ovariectomized rat model,and this effect may be related to the activation of the Wnt/β-catenin signaling pathway.

关 键 词：电针 脂肪干细胞 骨质疏松症 WNT/Β-CATENIN信号通路 

分 类 号：R245[医药卫生—针灸推拿学] R589.5[医药卫生—中医临床基础]