大豆光合限制作用的土壤水-氮交互响应及其与叶片水分利用效率的关系  

Photosynthetic limitation in soybean in response to soil water-nitrogen interactions and its relationship with leaf water use efficiency

作　　者：朱凯 范颖璇 左齐慧 谭思雨 刘奋武[1,2] 张健[1] 秦俊梅[1] 高丽娜 Zhu Kai;Fan Yingxuan;Zuo Qihui;Tan Siyu;Liu Fenwu;Zhang Jian;Qin Junmei;Gao Lina(Shanxi Agricultural University,Taigu,Shanxi 030801,China;State Key Laboratory of Sustainable Dryland Agriculture(in preparation),College of Resources&Environment of Shanxi Agricultural University,Taiyuan 030031,China)

机构地区：[1]山西农业大学,山西太谷030801 [2]山西农业大学资源环境学院,省部共建有机旱作农业国家重点实验室(筹),太原030031

出　　处：《植物科学学报》2024年第5期634-643,共10页Plant Science Journal

基　　金：山西省基础研究计划(自由探索类)项目(20210302124229);山西省高等学校科技创新项目(2021L156);山西农业大学科技创新基金项目(2020BQ70);山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目(202001-9);山西省博士毕业生、博士后研究人员来晋工作奖励资金科研项目(SXBYKY2021013)。

摘　　要：本文以我国北方旱地大豆(Glycine max(L.)Merr.)为材料,以光合限制作用为研究对象,设计土壤水-氮交互实验,探究其光合限制作用的水-氮交互响应及其与叶片瞬时水分利用效率(WUEins)和内在水分利用效率(WUEint)的相关性。结果显示:(1)大豆叶内CO_(2)扩散性限制作用(叶肉限制,l_(m);气孔限制,l_(sc))随水分胁迫逐渐增大,生化限制(l_(b))逐渐减小;(2)土壤水-氮交互后,植株l_(m)和l_(sc)均减小,而l_(b)显著增大;(3)l_(m)和l_(b)分别与WUEins及WUEint呈显著的负、正相关(P<0.05),而l_(sc)与二者的相关性不显著;(4)相较l_(sc)和l_(b),l_(m)对植株光合碳同化与叶片水分利用效率的贡献率最大,为大豆光合与水分利用能力变化的主导限制因子。研究结果旨在揭示土壤干旱与大气氮沉降双因子交互调控作物光合碳同化的生态效应,并分析该效应与作物水分利用能力的内在关系。Glycine max(L.)Merr.in northern China were selected as the experimental materials,with photosynthetic limitation as the primary focus of this study.A soil water-nitrogen interaction experiment was designed to explore the relationship between photosynthetic limitation and leaf instantaneous(WUEins)and intrinsic water use efficiency(WUEint).Results showed that:(1)CO_(2)diffusion limitations,including mesophyll limitation(l_(m))and stomatal limitation(l_(sc)),gradually increased with water stress,while biochemical limitation(l_(b))gradually decreased.(2)The interaction between water and nitrogen reduced l_(m)and l_(sc),while l_(b)significantly increased.(3)l_(m)and l_(b)were negatively and positively correlated with WUEins and WUEint,respectively(P<0.05),while l_(sc)showed no significant correlation with either.(4)Among the limiting factors,l_(m)contributed most to soybean photosynthetic carbon assimilation and leaf water use efficiency,making it the dominant constraint on photosynthetic and water use capacity.These findings reveal the ecological effects of soil drought and atmospheric nitrogen deposition on crop photosynthetic carbon assimilation and their intrinsic relationship with water use capacity.

关 键 词：水分利用效率 CO_(2)扩散性限制 生化限制 水分胁迫 氮添加 

分 类 号：Q945.79[生物学—植物学]