基于16S rDNA测序分析毛滴虫对猕猴肠道菌群结构的影响  

Effect of Trichomonas on the Structure of Macaca mulatta Gut Microbiota Based on 16S rDNA Sequencing Technology

作　　者：李鹤龄 陈智岗 宗发梁 宁卿 严玉霖 王宏 LI Heling;CHEN Zhigang;ZONG Faliang;NING Qing;YAN Yulin;WANG Hong(Institute of Primate Translational Medicine,Kunming University of Science and Technology,Kunming 650500,China;Yunnan Key Laboratory of Primate Biomedical Research,Kunming 650217,China;College of Veterinary Medicine,YunnanAgricultural University,Kunming 650201,China)

机构地区：[1]昆明理工大学灵长类转化医学研究院,昆明650500 [2]云南中科灵长类生物医学重点实验室,昆明650217 [3]云南农业大学动物医学院,昆明650201

出　　处：《中国畜牧兽医》2024年第11期4974-4983,共10页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31960121);云南省自然科学基金项目(202001BC070001、202102AA100053)。

摘　　要：【目的】通过16S rDNA测序技术分析毛滴虫感染对猕猴肠道菌群结构的影响,为临床研究利用微生物替代抗生素治疗毛滴虫病提供参考依据。【方法】分别收集毛滴虫阳性和阴性的猕猴新鲜粪便样本各6份,通过宏基因组测序进行肠道菌群和功能基因预测分析。【结果】与健康猕猴相比,感染毛滴虫的猕猴肠道菌群的多样性降低,菌群组成发生显著变化,拟杆菌和纤维杆菌显著减少(P<0.05),而弯曲菌和脱硫杆菌显著增加(P<0.05);肠道微生物中乳酸杆菌目(Lactobacillales)、弯曲杆菌属(Campylobacter)、弯曲杆菌科(Campylobacteraceae)等在感染毛滴虫的猕猴中显著富集,而普雷沃菌9菌(Prevotella_9)、拟杆菌目(Bacteroidales)、拟杆菌门(Bacteroidota)等在健康猕猴中更丰富;Brachyspira_hampsonii、Prevotellaceae_bacterium_Marseille_P2831是猕猴毛滴虫感染的生物标记物;肠道菌群功能基因预测显示,与氨基酸的生物合成和抗生素的生物合成途径相关的微生物基因功能在毛滴虫感染的猕猴中显著降低(P<0.05)。【结论】毛滴虫感染猕猴可降低肠道菌群的多样性,改变了肠道微生物群的结构,降低肠道菌群的生物合成功能。【Objective】This experiment was aimed to study the changes and diversity of gut microbiota in rhesus monkeys(Macaca mulatta)infected with Trichomonas using 16S rDNA sequencing technology,provide a reference for clinical research on using microbiota as an alternative antibiotics for treating trichomoniasis.【Method】Six samples of rhesus monkeys infected with Trichomonas and six samples of Trichomonas-negative fresh feces from rhesus monkeys were collected for predictive analysis of gut microbiota and functional genes through metagenomic sequencing.【Result】The diversity of gut microbiota in Trichomonas-infected rhesus monkeys decreased significantly compared to healthy rhesus monkeys,leading to significant changes in microbial community composition.Specifically,there was a significant decrease in Bacteroidota and Fibrobacterota(P<0.05),while Campylobacta and Desulfobacterota showed a significant increase(P<0.05).Additionally,Lactobacillales,Campylobacter and Campylobacteraceae were significantly enriched in the intestinal microbes of Trichomonas-infected rhesus monkeys,whereas Prevotella_9,Bacteroidales and Bacteroidota were significantly enriched in the intestinal microbes of Trichomonas-negative rhesus monkeys.Furthermore,Brachyspira_hampsonii and Prevotellaceae_bacterium_Marseille_P2831 were identified as biomarkers of Trichomonas-infected rhesus monkeys.The functional gene prediction of gut microbiota also revealed a significant decrease in the biosynthesis function of amino acids and antibiotics in the Trichomonas-infected rhesus monkeys(P<0.05).【Conclusion】Infection with Trichomonas could lead to a reduction in the diversity of gut microbiota and an alteration in the composition of the microbial community in rhesus monkeys.Additionally,it could also decrease the biosynthetic function of gut microbiota.

关 键 词：猕猴 毛滴虫 16S rDNA 肠道菌群 功能基因预测 

分 类 号：S852.72[农业科学—基础兽医学]