液相色谱-三重四极杆质谱法测定植物茶饮料中13种降糖类药物  

Determination of 13 Kinds of Hypoglycemic Drugs in Plant Beverages by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：陈文彬 程洁 张莎莎 周怡 蔡菲菲 李央央 张思思 周建峰 CHEN Wenbin;CHENG Jie;ZHANG Shasha;ZHOU Yi;CAI Feifei;LI Yangyang;ZHANG Sisi;ZHOU Jianfeng(The Comprehensive Technical Service Center of Wenzhou Customs,Wenzhou 325000;Wenzhou Technical Testing Research Institute for Quality,Wenzhou 325000)

机构地区：[1]温州海关综合技术服务中心,浙江温州325000 [2]温州市质量技术检测科学研究院,浙江温州325000

出　　处：《分析科学学报》2024年第5期553-558,共6页Journal of Analytical Science

基　　金：浙江省市场监督管理局科技计划项目(ZC2021B069)。

摘　　要：建立了液相色谱-三重四极杆质谱法同时测定植物茶饮料中13种降糖类药物含量的方法。样品经甲醇溶液超声提取10min后,定容至10.0mL,过0.22μm有机相滤膜,所得滤液采用Angilent Zorbax-Eclipse Plus C_(18)色谱柱分离,以不同体积比的乙腈和0.1%(体积分数)甲酸(含5mmol/L乙酸铵)水溶液进行梯度洗脱,质谱检测采用电喷雾离子源(ESI+)模式和多反应监测(MRM)模式,外标法定量。结果表明,13种降糖类药物标准曲线的线性范围为0.2~10.0ng/mL,定量限(LOQ)为2.0μg/kg,按标准加入法进行样品加标回收测试,回收率范围为85.9%~97.5%,测定值的标准偏差均≤5.87%。A liquid chromatographytandem mass spectrometry method has been developed for the simultaneous determination of 13 hypoglycemic drugs in plant beverages.The analytes in samples were extracted with methanol for 10 minutes by ultrasonic extraction.The target components were separated by an Angilent Zorbax Eclipse Plus C_(18)chromatographic column,and the gradient elution was carried out with different volume ratios of acetonitrile and 0.1%(volume fraction)formic acid(containing 5 mmol/L ammonium acetate)aqueous solution.The electrospray ionization in positive ion mode was used for analysis in multiple reaction monitoring(MRM)mode,and an external standard method was used for quantitative analysis.The result showed that the linear range of the standard curves for 13 hypoglycemic drugs were 0.2-10.0 ng/mL,with a limit of quantification(LOQ)of 2.0μg/kg.Standard addition method was used for sample recovery testing,with a recovery rate range of 85.9%-97.5%.The standard deviation of the measured values were less than 5.87%.

关 键 词：植物茶饮料 降糖类药物 液相色谱-质谱法 

分 类 号：O657.63[理学—分析化学]