高效液相色谱法测定番茄果肉中4种天然类胡萝卜素含量  

Determination of Four Natural Carotenoids in Tomato Flesh by High Performance Liquid Chromatography

作　　者：王楠[1] 周宝成[1] 葛志伟 吴筱丹 张志军 WANG Nan;ZHOU Baocheng;GE Zhiwei;WU Xiaodan;ZHANG Zhijun(School of Chemistry and Chemical Engineering,Zhejiang Sci-Tech University,Hangzhou,310018;Analysis Center of Agrobiology and Environmental Sciences of Zhejiang University,Hangzhou,310058)

机构地区：[1]浙江理工大学化学与化工学院,浙江杭州310018 [2]浙江大学农生环测试中心,浙江杭州310058

出　　处：《分析科学学报》2024年第5期592-596,共5页Journal of Analytical Science

基　　金：国家自然科学基金青年项目(22007083)。

摘　　要：建立了一种高效液相色谱法测定番茄果肉中4种亲脂性天然类胡萝卜素的方法。该方法选择YMCC_(30)色谱柱,以甲醇、乙腈和异丙醇为流动相,对番茄果肉中叶黄素、玉米黄素、α-胡萝卜素和β-胡萝卜素4种色素的含量测定进行了方法验证。在优化的色谱条件下,4种亲脂性天然类胡萝卜素线性相关性良好,相关系数(R^(2))均高于0.9980;检出限(LOD)在0.05~0.10μg/mL之间,定量限(LOQ)在0.10~0.30μg/mL之间;回收率在85.5%~112.4%之间。该方法操作简单、准确、可靠,适合蔬菜水果中天然类胡萝卜素含量分析。A new method for the quantitative determination of four lipophilic natural carotenoids in tomato pulp by high performance liquid chromatography(HPLC)was established.This method used YMC C_( 30) chromatographic column as the stationary phase,and methanol,acetonitrile and isopropanol as the mobile phase.The methodology has been validated by the determination of lutein,zeaxanthin,αcarotene andβcarotene in tomato pulp.Under this chromatographic condition,the four natural carotenoids had a high linear correlation,the correlation coefficient(R^( 2))was higher than 0.9980,the detection limit(LOD)of the four colorants was between 0.05 and 0.1μg/mL,the limit of quantification(LOQ)was in the range of 0.1-0.3μg/mL,and the recovery rate of the four pigments was all within 85.50%-112.4%.The method has the advantages of high flow sensitivity,good repeatability,high recovery rate and high instrument penetration rate.It is suitable for the analysis of natural carotenoids in fruits and vegetables.

关 键 词：高效液相色谱 类胡萝卜素 番茄果肉 

分 类 号：O657.72[理学—分析化学]