光周期处理对刺槐无性系组培苗生根的影响  

Effect of photoperiod treatment on rooting of tissue culture plantlets of Robinia pseudoacacia clonal

作　　者：张曼 伍越 孙宇涵[1,2,3,4,5] 彭祚登 张淑敏 于立新[6] 李云 ZHANG Man;WU Yue;SUN Yuhan;PENG Zuodeng;ZHANG Shumin;YU Lixin;LI Yun(College of Biological Science and Technology,Beijing Forestry University,Beijing 100083;College of Forestry,Beijing Forestry University,Beijing 100083;National Key Laboratory of Forest Tree Genetics and Breeding,Beijing Forestry University,Beijing 100083;Robinia Engineering Research Center,National Forestry and Grassland Administration,Beijing Forestry University,Beijing 100083;National Engineering Research Center for Forest Breeding and Ecological Restoration,Beijing Forestry University,Beijing 100083,China;Pingquan State-owned Loess Liangzi Forest Farm,Pingquan 067500,Hebei,China)

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]北京林业大学林学院,北京100083 [3]北京林业大学林木遗传育种全国重点实验室,北京100083 [4]北京林业大学国家林业和草原局刺槐工程技术研究中心,北京100083 [5]北京林业大学林木育种生态修复国家工程研究中心,北京100083 [6]河北省平泉市国有黄土梁子林场,河北平泉067500

出　　处：《中南林业科技大学学报》2024年第9期18-28,共11页Journal of Central South University of Forestry & Technology

基　　金：国家自然科学基金项目(31971675);国家重点研发计划项目(2017YFD0600503)。

摘　　要：【目的】研究不同光周期对刺槐无性系组培苗不定根发生的影响,分析不同光周期下根原基突出时间及不同阶段根系的生长状态和生理指标,探讨其生根能力,筛选出促进刺槐生根的最适光周期。【方法】以4个二倍体刺槐无性系组培苗为材料,设置5种光周期处理,统计分析不同光周期下刺槐生根率及生理指标变化,并观察根外部形态变化,同时每天取根基部制作石蜡切片,观察根原基发生时间。【结果】1)解剖学观察显示,刺槐无性系组培苗生根过程分为3个时期:愈伤组织形成期(0~7 d)、不定根形成期(根原基诱导期)(7~10 d)、不定根伸长期(10 d后)。2)生根情况及解剖学观察结果显示,4个刺槐无性系中,D26-5①刺槐无性系根原基较早出现,同时生根能力最强,生根率为92%,且24 h/0 h光周期处理下根原基突出时间最早,生根率提高至97.8%。3)生根过程中,可溶性糖和叶绿素含量随着光周期增加而上升,24 h/0 h光周期下含量最高。丙二醛含量呈现出下降—上升—下降的趋势,脯氨酸含量呈先上升再下降的趋势,两者在24 h/0 h光周期处理下的含量都要小于16 h/8 h光周期,说明刺槐应对连续光照时可通过调节渗透物质等方式适应环境的变化。【结论】刺槐无性系组培苗不定根发生能力受光周期影响,在24 h/0 h光周期下根原基突出时间最早,生根能力最强。【Objective】The effect of different photoperiod on the occurrence of adventitious roots of black locust clones was studied,and the growth state and physiological indexes of roots at different stages under different photoperiod were analyzed,and the rooting ability was discussed to select the photoperiod that most promoted the rooting of black locust clones.【Method】Five photoperiodic treatments were applied to 4 diploid black locust clones.The rooting rate and physiological indexes of black locust were statistically analyzed under different photoperiodic conditions,and the morphological changes outside the roots were observed.At the same time,paraffin sections were taken from the base every day to observe the occurrence time of root primordia.【Result】1)Anatomical observations show that the rooting process of the tissue culture seedlings of black locust clones can be divided into three stages:callus formation stage(0-7 d),adventitious root formation stage(root induction stage)(7-10 d),adventitious root elongation stage(after 10 d).2)The results of rooting and anatomical observation showed that among the 4 black locust clones,the root primordia of D26-5①appeared earlier and had the strongest rooting ability(rooting rate was 92%),and the root primordia protrusion time was the earliest under 24 h photoperiod treatment,and rooting rate increased to 97.8%.3)During the rooting process,the contents of soluble sugar and chlorophyll increased with the increase of photoperiod,and the contents were the highest at 24 h photoperiod.The content of malondialdehyde(MDA)decreased,and the content of proline(PRO)increased first and then decreased,and the content of both of them was lower in 24 h photoperiod than in 16 h photoperiod.These results indicate that plants can resist environmental changes by adjusting osmotic substances in response to continuous light.【Conclusion】The ability of adventitious root generation is affected by photoperiod.The root primordium protrusion time is the earliest and the rooting ability

关 键 词：刺槐 光周期 不定根 石蜡切片 

分 类 号：S718.43[农业科学—林学]