蒿叶猪毛菜GPAT基因家族成员鉴定及其在干旱胁迫下的表达模式分析  

Identification of GPAT Gene Family Members in Salsola abrotanoides and Their Expression Analysis under Drought Stress

作　　者：杜明阳 马健芝 多杰措 罗天蓉 熊辉岩[2] 陈亦婷 段瑞君 DU Mingyang;MA Jianzhi;DUO Jiecuo;LUO Tianrong;XIONG Huiyan;CHEN Yiting;DUAN Ruijun(College of Eco-Environmental Engineering,Qinghai University,Xining 810000;College of Agriculture and Animal Husbandry,Qinghai University,Xining 810000)

机构地区：[1]青海大学生态环境工程学院,西宁810000 [2]青海大学农牧学院,西宁810000

出　　处：《植物遗传资源学报》2024年第10期1781-1792,共12页Journal of Plant Genetic Resources

基　　金：青海省中央引导地方科技发展资金计划项目(2023ZY003);2023年度青海省“昆仑英才·高端创新创业人才”计划项目~~。

摘　　要：甘油-3-磷酸酰基转移酶(GPAT,glycerol-3-phosphate acyltransferase)是三酰甘油(TAG,triacylglycerol)生物合成的核心酶,在植物生长发育以及抗逆过程中发挥重要作用。本研究基于蒿叶猪毛菜(Salsola abrotanoides L.)转录组数据,利用生物信息学和q RT-PCR方法,对蒿叶猪毛菜GPAT基因家族成员进行鉴定分析并探究其表达模式。以BLASTP比对和HMM搜索2种方法共鉴定出35个GPAT成员;理化性质分析表明,大多数蛋白属于碱性蛋白;亚细胞定位预测表明,Sa_GPAT家族成员主要分布于内质网;空间结构预测显示,二级结构主要以α螺旋为主,三级结构较为稳定。系统发育树分析显示,该家族分为4个亚家族,其中组别4为特有亚家族,且与拟南芥GPAT家族不存在聚类关系。信号肽预测显示蒿叶猪毛菜GPAT家族成员中未存在信号肽,且跨膜结构分析表明蒿叶猪毛菜GPAT家族成员中约63%存在跨膜结构。表达模式分析表明,蒿叶猪毛菜GPATs呈现组织特异性表达,其中Sa_GPAT003在幼苗中高度表达,结合幼苗干旱胁迫转录组结果发现多数Sa_GPAT家族成员响应外界干旱胁迫,其中Sa_GPAT032在干旱胁迫后表达量极显著升高。由此推测,蒿叶猪毛菜GPAT基因家族成员可能在抵抗干旱胁迫方面起着重要作用。Based on the genome and transcriptome data of Salsola abrotanoides,we identified and analysed the GPAT gene family members of Salsola abrotanoides and explored their transcriptional patterns under drought stress treatment using bioinformatics and qRT-PCR methods.A total of 35 GPAT members,named Sa_GPAT1-35,were identified by BLASTP comparison and HMM search,and most of them encoded to the basic proteins that were mainly localized to the endoplasmic reticulum using the bioinformatics prediction.Spatial structure prediction showed that the secondary structure of Sa_GPAT protein was mainly dominated byα-helix with stable tertiary structure.These family members were divided into four subfamilies,of which subfamily 4 of Salsola abrotanoides was unique and not found in other species such as Arabidopsis.No signal peptides were found by prediction,and approximately 63%of Salsola abrotanoides GPAT family members were predicted with transmembrane structure.Through real time fluorescence quantitative PCR analysis,the Salsola abrotanoides GPATs genes were detected showing tissue-specific transcriptional characteristics.Sa_GPAT003 was highly expressed in seedlings.In conjugation with transcriptome results under drought stress treatment,most family members were found with changed profiles.Sa_GPAT032 showed significantly higher expression after drought stress treatment.The comprehensive analysis results suggest that GPAT gene family members may play an important role in drought stress response in Salsola abrotanoides.

关 键 词：蒿叶猪毛菜 GPAT基因家族 生物信息学 转录组 

分 类 号：Q943.2[生物学—植物学]