低强脉冲超声联合聚维酮碘溶液对耐甲氧西林金黄色葡萄球菌生物膜的体外抗菌活性研究  

Antibacterial effects in vitro of low-intensity pulsed ultrasound combined with 3.5 g/L povidone-iodine on the biofilm of methicillin-resistant staphylococcus aureus

作　　者：王添兴 李国庆[1] 汪洋[1] 纪保超[1] 陈永杰 周海康 曹力[1] Wang Tianxing;Li Guoqing;Wang Yang;Ji Baochao;Chen Yongjie;Zhou Haikang;Cao Li(Department of Joint Surgery,The First Hospital Affiliated to Xinjiang Medical University,Institute of High Incidence Diseases Research in Xinjiang Region,A Key Laboratory of the Ministry of Education(Xinjang Medical University),Xinjiang Clinical Research Center for Orthopedics,Urumqi 830011,China)

机构地区：[1]新疆医科大学第一附属医院关节外科,新疆地区高发疾病研究教育部重点实验室(新疆医科大学),新疆骨科疾病临床医学研究中心,乌鲁木齐830011

出　　处：《中华创伤骨科杂志》2024年第9期818-823,共6页Chinese Journal of Orthopaedic Trauma

基　　金：新疆维吾尔自治区科技计划重大专项课题(2022A03011);新疆维吾尔自治区自然科学基金面上项目(2022D01C475)。

摘　　要：目的探讨低强脉冲超声联合3.5 g/L聚维酮碘溶液对耐甲氧西林金黄色葡萄球菌(MRSA)生物膜的体外抗菌活性。方法在玻璃爬片或共聚焦皿表面建立MRSA未成熟(培养24 h)及成熟(培养72 h)生物膜,根据不同干预方法随机分成4组(n=9):对照组将玻璃爬片或共聚焦皿置入500 mL生理盐水中3 min,PI组将玻璃爬片或共聚焦皿置入500 mL 3.5 g/L聚维酮碘溶液中3 min,LIPUS组将玻璃爬片或共聚焦皿置入500 mL生理盐水中同时联合低强脉冲超声(LIPUS)干预3 min,LIPUS&PI组将玻璃爬片或共聚焦皿置入500 mL 3.5 g/L聚维酮碘溶液中同时联合LIPUS干预3 min。干预后进行共聚焦显微镜(CLSM)、扫描电镜(SEM)观察,比较4组MRSA生物膜的结构、形态、生物膜内细菌存活状态及活菌计数等。结果在培养24、72 h生物膜中,CLSM及SEM观察到LIPUS组及LIPUS&PI组生物膜稀疏,且LIPUS&PI组伴有大量死菌。在培养24 h生物膜中,对照组、PI组、LIPUS组及LIPUS&PI组的细菌菌落数分别为(1.21±0.45)×10^(6)、(3.38±2.81)×10^(3)、(1.82±0.37)×10^(3)、(69.67±27.93)CFU/mL,除PI组与LIPUS组比较差异无统计学意义(P>0.05)外,其余各组之间两两比较差异均有统计学意义(P<0.05)。在培养72 h生物膜中,对照组、PI组、LIPUS组及LIPUS&PI组的细菌菌落数分别为(3.01±0.70)×10^(6)、(1.80±1.52)×10^(5)、(2.10±0.52)×10^(3)、(68.67±19.55)CFU/mL,4组之间两两比较差异均有统计学意义(P<0.05)。结论单独LIPUS或3.5 g/L聚维酮碘溶液在未成熟及成熟体外生物膜中作用3 min仅具有部分抗菌活性。LIPUS可以增强3.5 g/L聚维酮碘溶液在不同成熟度体外生物膜中的抗菌活性。Objective To explore the in vitro antibacterial effects of low-intensity pulsed ultrasound(LIPUS)combined with 3.5 g/L povidone iodine solution on the biofilm of methicillin-resistant staphylococcus aureus(MRSA).Methods Immature(cultured for 24 hours)and mature(cultured for 72 hours)MRSA biofilms were established on the surfaces of glass slides or confocal dishes.They were randomly divided into 4 groups(n=9)according to different intervention methods.In the control group,glass slides or confocal dishes were placed in 500 mL of physiological saline for 3 minutes;in the PI group,glass slides or confocal dishes were placed in 500 mL of 3.5 g/L povidone iodine solution for 3 minutes;in the LIPUS group,glass slides or confocal dishes were placed in 500 mL of physiological saline and simultaneously intervened with LIPUS for 3 minutes;in the LIPUS&PI group,glass slides or confocal dishes were placed into 500 mL of 3.5 g/L povidone iodine solution and simultaneously intervened with LIPUS for 3 minutes.After intervention,confocal microscopy(CLSM)and scanning electron microscopy(SEM)were used to observe and compare the structure,morphology,bacterial survival,and viable cell count of the MRSA biofilms among the 4 groups.Results On the MRSA biofilms cultured for 24 and 72 hours,CLSM and SEM observed sparse biofilms in the LIPUS group and LIPUS&PI group,and also a large number of dead bacteria in the LIPUS&PI group.On the MRSA biofilms cultured for 24 hours,the bacterial colony counts in the control group,PI group,LIPUS group,and LIPUS&PI group were(1.21±0.45)×10^(6)CFU/mL,(3.38±2.81)×10^(3)CFU/mL,(1.82±0.37)×10^(3)CFU/mL,and(69.67±27.93)CFU/mL,respectively.Except for the comparison between PI group and LIPUS group,which showed no statistically significant difference(P>0.05),there were statistically significant differences between the other groups when compared pairwise(P<0.05).On the MRSA biofilms cultured for 72 hours,the bacterial colony counts in the control group,PI group,LIPUS group,and LIPUS&PI group were(3.01±

关 键 词：聚维酮碘 生物膜 低强脉冲超声 耐甲氧西林金黄色葡萄球菌 抗菌活性 

分 类 号：R378.11[医药卫生—病原生物学]