江西嗜酸链霉菌去铁胺E及金属络合物的分离鉴定与生物活性研究  

Study on Isolation,Identification and Bioactivity of Desferrioxamine E and Its Metal-Complexes from Streptacidiphilus jiangxiensis

作　　者：李金达 程伯涛 黄积武 刘文[2] Li Jinda;Cheng Botao;Huang Jiwu;Liu Wen(College of Chemistry and Materials Science,Shanghai Normal University,Shanghai 200233;State Key Labratory of Chemical Biology,Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai 200032)

机构地区：[1]上海师范大学化学与材料科学学院,上海200233 [2]中国科学院上海有机化学研究所、生命过程小分子调控全国重点实验室,上海200032

出　　处：《有机化学》2024年第7期2377-2380,共4页Chinese Journal of Organic Chemistry

基　　金：科技部重大专项(No.2022YFC2303100);国家自然科学基金(Nos.22193070,32030002)资助项目。

摘　　要：嗜酸链霉菌属细菌的研究主要集中在新物种的分离和鉴定上,而其次级代谢产物的研究则相对较少.从江西嗜酸链霉菌的发酵液中分离得到一个新的铝配合物铝草铵(alumioxamine,3)和两个已知化合物去铁胺E(desferrioxamine E,1)和铁草铵E(ferrioxamine E,2),并通过MS、IR、NMR以及单晶X射线衍射技术对其结构进行鉴定.从结构上来看,化合物3以铝为中心,与配体的六个O原子相连,形成六齿配合物.此外,体外细胞毒活性实验表明,在100μmol/L浓度时化合物1对4种人肿瘤细胞(Hela、PANC1、A375和MHCC-97H)均具有一定的抑制活性,细胞存活率为4.8%~66.4%.Research on the genus Streptacidiphilus was mainly focused on the isolation and identification of new species,with relatively less emphasis on the study of secondary metabolites.A new aluminium-complex alumioxamine(3),and two known compounds desferrioxamine E(1)and ferrioxamine E(2)were isolated from the fermentation broth of Streptacidiphi-lus jiangxiensis CGMCC 4.1857.Their structures were elucidated by MS,IR,NMR and single crystal X-ray diffraction tech-nologies.Structurally,compound 3 features aluminum(III)as the central atom,which is bonded to six oxygen atoms of the ligand,forming a hexadentate complex.Furthermore,in vitro cytotoxicity assays showed that compound 1 exhibited potential inhibitory activity against four human tumor cell lines(Hela,PANC1,A375 and MHCC-97H)at a concentration of 100μmol/L with cell viability ranging from 4.8%to 66.4%.

关 键 词：江西嗜酸链霉菌 次级代谢产物 配合物 细胞毒活性 晶体结构 

分 类 号：Q939.9[生物学—微生物学] O641.4[理学—物理化学]