益心通痹合剂干预动脉粥样硬化的机制研究  

Study on mechanism of Yixin Tongbi Mixture in intervention of atherosclerosis

作　　者：聂谦[1] 赵珏[1] 韩明君 张宏才[1] 张德来[1] 谢文[1] NIE Qian;ZHAO Jue;HAN Mingjun;ZHANG Hongcai;ZHANG Delai;XIE Wen(Department of Cardiology,Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan 610000,China)

机构地区：[1]成都中医药大学附属医院心内科,成都610000

出　　处：《重庆医学》2024年第20期3048-3054,共7页Chongqing Medical Journal

基　　金：国家重点研发计划“中医药现代化研究”重点专项项目(2017YFC1700403);四川省中医药管理局科学技术研究专项项目(2024MS165);四川省中医医院基金项目(20ZJ08)。

摘　　要：目的探讨益心通痹合剂干预动脉粥样硬化(AS)的相关分子机制。方法将36只SD大鼠分为正常对照组、模型对照组、低剂量益心通痹合剂组、中剂量益心通痹合剂组、高剂量益心通痹合剂组及阿托伐他汀组,每组6只,除正常对照组外其余组采用高脂维生素D3混合饲料灌胃法建立大鼠AS模型。低、中、高剂量益心通痹合剂组大鼠分别灌胃10、20、40 g/kg的益心通痹合剂,阿托伐他汀组灌胃5 mg/kg阿托伐他汀,正常对照组和模型对照组大鼠灌胃安慰剂,1次/d,连续干预14 d。采用HE染色观察大鼠主动脉组织的病理变化,全自动生化分析仪检测大鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)及低密度脂蛋白(LDL)水平,ELISA检测主动脉组织白细胞介素(IL)-6、肿瘤坏死因子-α(TNF-α)和细胞间黏附分子-1(ICAM-1)表达,Western blot检测主动脉组织基质金属蛋白酶(MMP)-2、MMP-9和心肌组织B淋巴细胞瘤-2基因(Bcl-2)及相关X蛋白(Bax)表达。结果与正常对照组比较,模型对照组大鼠主动脉管壁明显增厚,血清TC、TG、HDL及LDL水平升高,主动脉组织MMP-2、MMP-9、IL-6、TNF-α及ICAM-1表达升高,心肌组织Bax表达升高、Bcl-2表达降低,差异有统计学意义(P<0.05)。与模型对照组比较,中、高剂量益心通痹合剂组和阿托伐他汀组上述指标均有明显改善(P<0.05),低剂量益心通痹合剂组除Bax、IL-6、ICAM-1表达外其他指标均无差异(P>0.05)。结论益心通痹合剂可能通过抑制炎症及细胞凋亡来抑制AS的发生、发展,其机制与IL-6/TNF-α和MMP-2/MMP-9表达异常有关。Objective To explore the molecular mechanism of Yixin Tongbi Mixture in the intervention of atherosclerosis(AS).Methods Thirty-six SD rats were divided into the normal control group,model control group,low dose Yixin Tongbi Mixture group,medium dose Yixin Tongbi Mixture group,high dose Yixin Tongbi Mixture group and atorvastatin group,6 cases in each group.Except the normal control group,the other groups adopted the high fat vitamin D3 mixed feed gavage for constructing the rat AS model.The low dose,medium dose and high doses Yixin Tongbi Mixture groups were gavaged by 10,20,40 g/kg Yixin Tongbi Mixture.The atorvastatin group was gavaged by 5 mg/kg atorvastatin;and the normal control group and model control group were gavaged by placebo once a day for consecutive 14 d.The pathological changes of aortic tissues were observed by HE staining.The levels of serum TC,TG,HDL and LDL were detected by automatic biochemical analyzer.The expression levels of IL-6,TNF-αand ICAM-1 in aortic tissue were detected by ELISA.The expression levels of MMP-2 and MMP-9 in aortic tissue and Bax and Bcl-2 protein expression levels in cardiac tissue were detected by Western blot.Results Compared with the normal control group,the aortic wall of the model control group rats was significantly thickened,and the levels of serum TC,TG,HDL and LDL were increased.The expression levels of MMP-2,MMP-9,IL-6,TNF-αand ICAM-1 in the aortic tissues were also increased.The expression level of Bax in the myocardial tissues was increased,while the expression level of Bcl-2 was decreased,and the differences were statistically significant(P<0.05).Compared with the model control group,the above indicators in the medium dose and high doses Yixin Tongbi Mixture groups and atorvastatin group were significantly improved(P<0.05).Except Bax,IL-6 and ICAM-1 expression levels,the other indicators in the low dose Yixin Tongbi Mixture group had no statistically significant differences(P>0.05).Conclusion Yixin Tongbi Mixture could inhibit the AS occurrence and develo

关 键 词：益心通痹合剂 动脉粥样硬化 炎症因子 基质金属蛋白酶 

分 类 号：R259[医药卫生—中西医结合]