The transition from 2G to 3G-feedstocks enabled efficient production of fuelsand chemicals  

作　　者：Kai Wang Changsheng Su Haoran Bi Changwei Zhang Di Cai Yanhui Liu Meng Wang Biqiang Chen Jens Nielsen Zihe Liu Tianwei Tan 

机构地区：[1]College of Life Science and Technology,Beijing University of Chemical Technology,Beijing Advanced Innovation Center for Soft Matter Science and Engineering,No.15 North 3rd Ring Rd East,Beijing,100029,China [2]National Energy R&D Center for Biorefnery,Beijing Key Lab of Bioprocess,Beijing University of Chemical Technology,No.15 North 3rd Ring Rd East,Beijing,100029,China [3]Department of Life Sciences,Chalmers University of Technology,Gothenburg,SE41296,Sweden

出　　处：《Green Energy & Environment》2024年第11期1759-1770,共12页绿色能源与环境（英文版）

基　　金：supported by the National Key R&D Program of China[2021YFC2103500];National Natural Science Foundation of China(22211530047);Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project[grant numbers:TSBICIP-KJGG-009];the Beijing Advanced Innovation Center for Soft Matter Science and Engineering,Beijing University of Chemical Technology.

摘　　要：For decades micoorganisms have been engineered for the utilization of lignocellulose-based second-generation (2G) feedstocks, but with theconcerns of increased levels of atmospheric CO_(2) causing global warming there is an emergent need to transition from the utilization of 2Gfeedstocks to third-generation (3G) feedstocks such as CO_(2) and its derivatives. Here, we established a yeast platform that is capable ofsimultaneously converting 2G and 3G feedstocks into bulk and value-added chemicals. We demonstrated that by adopting 3G substrates such asCO_(2) and formate, the conversion of 2G feedstocks could be substantially improved. Specifically, formate could provide reducing power andenergy for xylose conversion into valuable chemicals. Simultaneously, it can form a concentrated CO_(2) pool inside the cell, providing thermodynamically and kinetically favoured amounts of precursors for CO_(2) fixation pathways, e.g., the Calvin–Benson–Bassham (CBB) cycle.Furthermore, we demonstrated that formate could directly be utilized as a carbon source by yeast to synthesize endogenous amino acids. Theengineered strain achieved a one-carbon (C1) assimilation efficiency of 9.2%, which was the highest efficiency observed in the co-utilization of2G and 3G feedstocks. We applied this strategy for productions of both bulk and value-added chemicals, including ethanol, free fatty acids(FFAs), and longifolene, resulting in yield enhancements of 18.4%, 49.0%, and ~100%, respectively. The strategy demonstrated here for coutilization of 2G and 3G feedstocks sheds lights on both basic and applied research for the up-coming establishment of 3G biorefineries.

关 键 词：TRANSITION utilized BENSON 

分 类 号：TQ072[化学工程] TQ517